LOCUS       sequence-283500-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1334
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             817..840
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tggccacggc ggcttctaac ccctacctgc cggggaacag cctgctcgcg
       61 gccggctcta ttgtgcactc ggacgcggca ggggctggcg gcggcggggg tggcggcggc
      121 ggcggcggcg ggggcggcgc agggggcggg ggcggcggca tgcagccggg cagcgccgcc
      181 gtgacctcgg gcgcctaccg gggggacccg tcctctgtca agatggtcca gagcgacttc
      241 atgcaggggg ccatggccgc cagcaacggc ggccatatgc tgagccacgc gcaccagtgg
      301 gtcacagccc tgccccacgc cgccgccgcc gccgccgctg ccgccgccgc cgccgtggag
      361 gcgagctcgc cgtggtcggg cagcgccgtg ggcatggctg gcagccccca gcagccaccg
      421 cagccgccgc cgccaccgcc gcagggcccc gacgtgaagg gcggcgccgg gcgcgacgac
      481 ctgcacgcgg gcacagcgct gcaccaccgc gggccgccgc acctcggacc cccgccgccg
      541 cccccacacc agggccaccc tgggggctgg ggggcggccg ccgctgccgc agccgcagcc
      601 gccgccgccg ccgccgccgc gcacctcccg tccatggccg ggggccagca gccgccgccg
      661 cagagtctgc tctactcgca gcccggaggc ttcacggtga acggcatgct gagcgcgcca
      721 ccggggcccg gcggcggcgg cggcggcgcg ggcggtggag cccagagctt ggtgcacccg
      781 gggctggtgc gcggggacac gccagagctg gccgagcacc accaccacca ccaccaccac
      841 gcgcatcctc acccgccgca cccgcaccac gcgcagggac ccccgcacca cggcggcggc
      901 ggcggcggcg cggggcctgg actcaacagc cacgacccgc actcggacga ggacacgccg
      961 acgtcggacg acctggagca gttcgccaag cagttcaagc agcggcgcat caagctgggc
     1021 ttcacgcagg ccgacgtggg gttggcgctg ggcacactct acggcaacgt gttctcgcag
     1081 accaccatct gccgcttcga ggccctgcag ctgagcttca agaacatgtg caagctcaag
     1141 ccgctgctga acaagtggct ggaggaggcg gactcaagca ccggcagccc cacaagcatc
     1201 gacaagatcg cggcgcaggg ccgcaagcgc aagaagcgga cctctatcga ggtgagcgtc
     1261 aagggcgcgc tggagagcca cttcctcaag tgccccaagc cctccgcgca ggagatcacc
     1321 aacctggccg acagcctgca gctcgagaag gaggtggtgc gggtctggtt ctgcaatcgg
     1381 cgccaaaagg agaagcgcat gacgccgccc gggatccaac agcagacgcc cgacgacgtc
     1441 tactcgcagg tgggcaccgt gagcgccgac acgccgccgc ctcaccacgg gctgcagacg
     1501 agcgttcagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga actggcatac acccaagtct aaac
//
