LOCUS       sequence-283503-        1481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1344
FEATURES             Location/Qualifiers
     source          1..1481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaagctc ggcttccctg gagaccatgg tgcccccggc ctgcccgcgc
       61 gccggagcgt cgccggccac ttccaagaca ctggcctttt ccatcgagcg catcatggcc
      121 aagacgtcgg agccccgtgc gccctttgag ccccggcctg gagcgctaga ggcggacggc
      181 agccagggca agaaactgct caacctctgc tcgccgctgc cctgtatgat ccccctccag
      241 cccctaggct acgaggtgcc gtcaaagaca ctgctcagtt actcggagct ctggaaaagc
      301 agcctccggg cgggcggcgg cggaggcggc ggcggcggtg gcggcggcgg cggcgggggg
      361 gccccagtgt gcggcgccag cggcttgtgc aaaaccaact gtggcgtgtg ctgcaaggcc
      421 gagctgggcc tggcgccgtc cgcgctgccc gcgggcaggg tcatcaagcc gcaggtcatc
      481 aaccaggctg tggggctgcc ggccagcggc tcgctctact acttcaacta cctggactcg
      541 accgcgtacc cgccgtctga gctcctcagc ggccacctct tcccgtctgg cctcctcaat
      601 gcgcaggccc ccgccgccct ggctgctcac cccaagctct ttctgctgga gaacgccaag
      661 ctggccggcc tggctgcgga caagttcccc cacccggctc cctatcccca taaggagcgc
      721 ttgccggcgc cgctggagca ggtactgaag gaaaactcgg ccctgactgc cgagcgcgga
      781 ggcgtcaagg gccacagcaa gctgccagga ggctccgcag atggcaagcc caaaaacttc
      841 acctgcgagg tgtgcggcaa ggtgtttaac gctcactata atctcacccg ccacatgccg
      901 gtccacaccg gagccagacc gttcgtgtgc aaagtctgcg gcaaaggctt tcgccaggcc
      961 agcacgctct gcaggcacaa aattatccac acccaggaaa agccacataa atgcaaccag
     1021 tgcggcaaag cgttcaaccg cagctccacg ctcaacacgc atatccgcat ccacgcgggc
     1081 tacaagccct tcgtctgcga attttgcggc aaaggctttc accaaaaagg gaactacaag
     1141 aaccacaagc tgacccacag cggcgagaag cagtacaaat gtaccatctg caacaaggcc
     1201 ttccaccagg tctacaacct aaccttccat atgcacaccc acaacgacaa gaagcctttc
     1261 acgtgcgcca cttgcggcaa agggttttgc agaaactttg acttaaagaa acatgtgcgc
     1321 aaactccacg acagcgtggg ccctgctgcc ccctccgcaa aggacctgac taggacagtg
     1381 cagagctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1441 tcttgtggaa aggacgagaa ccctttacga cgctacgcac c
//
