LOCUS       sequence-283512-        1250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1380
FEATURES             Location/Qualifiers
     source          1..1250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctgcg ggaagaagcc tggtctccgg ggccgctgga ttctgaggac
       61 cagcagatgg ccagtcacga gaacccagtg gacatcctca tcatggatga tgacgacgtc
      121 cccagctggc ctcccaccaa gctgtccccg ccccagtctg cgcccccagc cggcccgcct
      181 ccccggccgc ggccgcccgc cccctacatc tgcaacgagt gtggcaagag ctttagccac
      241 tggtccaagc tgacgcggca ccagcgcacg cacaccggcg agcggcccaa cgcctgcgcc
      301 gactgcggca agaccttctc gcagagctcg cacctggtgc agcaccggcg catccacacg
      361 ggcgagaagc cctacgcctg cttggagtgc ggcaagcgct tcagctggag ctccaacctc
      421 atgcagcacc agcgcatcca cacgggcgag aagccctaca cctgccccga ctgcggccgc
      481 agcttcactc agagcaagag cctggccaag caccggcgct cgcacagcgg cctcaagccc
      541 ttcgtgtgcc cgcgctgcgg ccgcggcttc agccagccca agagcctcgc gcgtcacctg
      601 cggctgcacc cggagctgtc ggggcctggc gtggcggcca aggtgctggc ggctagcgtc
      661 cggcgggcca aggggcccga agaggcggtg gcggcggacg gcgagatcgc catccccgtg
      721 ggcgatggcg agggcatcat cgtggtgggc gcgccgggcg agggggccgc ggccgcggca
      781 gccatggcgg gcgcgggggc aaaggccgcg gggccccggt cgcggcgcgc cccggccccc
      841 aagccgtacg tgtgtctgga gtgcgggaag ggcttcgggc acggggccgg gctcctggcg
      901 caccagcggg cccagcacgg ggacgggctc ggggcggcgg ggggcgagga gccggcccac
      961 atctgcgtgg agtgcgggga gggcttcgtg cagggcgccg cgctccggag acacaagaag
     1021 atccacgcgg tgggcgcgcc ctcggtctgc agcagctgcg gacagagcta ctaccgcgcg
     1081 ggcggggagg aggaggacga cgacgacgag gccgcgggcg ggcggtgccc cgagtgccgc
     1141 ggtggggagg gccggtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1201 ctttatatat cttgtggaaa ggacgaaatt taaccattaa actcgcatcc
//
