LOCUS       sequence-28352-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUB1
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..28
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     44..60
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             134..160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    176..200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
       61 tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg acgatgacaa
      121 gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt atcaaacaag
      181 tttgtacaaa aaagttggca tggcggcgga ggaacctcag cagcagaagc aggagccgct
      241 gggcagcgac tccgaaggtg ttaactgtct ggcctatgat gaagccatca tggctcagca
      301 ggaccgaatt cagcaagaga ttgctgtgca gaaccctctg gtgtcagagc ggctggagct
      361 ctcggtccta tacaaggagt atgctgaaga tgacaacatc tatcaacaga agatcaagga
      421 cctccacaaa aagtactcgt acatccgcaa gaccaggcct gacggcaact gtttctatcg
      481 ggctttcgga ttctcccact tggaggcact gctggatgac agcaaggagt tgcagcggtt
      541 caaggctgtg tctgccaaga gcaaggaaga cctggtgtcc cagggcttca ctgaattcac
      601 aattgaggat ttccacaaca cgttcatgga cctgattgag caggtggaga agcagacctc
      661 tgtcgccga
//
