LOCUS       sequence-283524-        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1400
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtgat gttggaaaac tattgccacc tcatctctgt ggggtgtcac
       61 atgaccaaac ctgatgtgat cctcaagttg gaacgaggag aagagccatg gacatcattt
      121 gcaggtcata cctgcttgga agaaaactgg aaagctgaag actttttagt gaaattcaag
      181 gaacaccaag agaagtattc tagatcagtt gtaagcatca accacaaaaa actggtgaag
      241 gagaagagta aaatatatga aaagacattt actctaggca aaaaccctgt gaattcaaaa
      301 aatctacctc ctgaatatga tactcatgga aggattttga aaaatgtttc agaattaatc
      361 atcagtaatc taaatcctgc aagaaagaga cttagtgagt ataatggata tgggaaatca
      421 ctcctgagta ctaaacaaga gactactcat cctgaagtca aatcccataa tcaaagtgcc
      481 agagctttca gtcataatga agttcttatg cagtatcaga aaacggaaac tccagcacag
      541 tcatttggat ataatgactg tgagaaatca ttccttcaaa ggggaggcct gattacacat
      601 agtagacctt acaaaggaga aaacccatct gtatataata aaaaaagaag agcaaccaat
      661 attgaaaaaa aacatacatg caatgaatgt gggaaatctt tctgcaggaa atcagtattg
      721 attctgcatc agggaattca ctcagaagaa aaaccctatc aatgtcatca atgtggaaat
      781 gcatttagaa ggaaatcata tctcattgat catcagagaa ctcacacagg agagaaaccc
      841 tttgtttgca atgaatgtgg taagtccttc cgcctcaaga cagccctcac tgatcatcag
      901 agaacacaca caggggagaa atcgtatgaa tgtctgcaat gtaggaatgc cttcagattg
      961 aagtcacacc tcattcgtca tcagagaact cacacgggag agaaaccata tgagtgtaat
     1021 gactgtggga agtccttccg ccagaagaca acactctctc tacatcagag aatccataca
     1081 ggtgagaaac cctacatttg taaagaatgt gggaagtcct ttcaccagaa ggcaaatctt
     1141 actgtacatc agagaactca tacaggggaa aagccctata tttgtaatga atgtgggaaa
     1201 tccttctccc agaagacaac ccttgctctt catgagaaaa ctcataatga ggagaaaccc
     1261 tatatttgta gtgaatgtgg aaagtccttc cgccagaaga caacccttgt agcacatcag
     1321 agaacacata caggggagaa atcttatgaa tgtcctcact gtgggaaggc ctttagaatg
     1381 aagtcatacc tcattgatca tcaccgaact cacacaggag agaaaccata tgaatgtaat
     1441 gaatgtggta aatcattcag tcaaaagaca aatctcaatc tacatcagag aattcataca
     1501 ggggagaaac cctatgtttg taatgaatgt gggaagtcct ttcgccagaa agcaaccctc
     1561 actgtacatc agaaaataca taccggccag aaatcctatg aatgtcctca gtgtgggaaa
     1621 gcctttagca ggaagtcata tctcattcat catcaaagaa ctcatacggg agagaaacca
     1681 tataaatgta gtgaatgtgg aaagtgcttc cgccagaaga caaatcttat tgtacatcag
     1741 agaactcaca caggtgagaa accctatgtt tgtaatgagt gtggtaagtc tttcagttat
     1801 aagagaaacc tcattgtcca tcaaagaact cacaagggag aaaacattga aatgcaatag
     1861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1921 aaggacgacc atcacggact agctccaatg at
//
