LOCUS       sequence-28354-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUD1
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..77
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     93..109
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgnnnnnnnt ctcccccttg
       61 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
      121 gcgccggaat tagatctgcc accatggact acaaggatga cgatgacaag ctcgatggag
      181 gatacccata cgatgttcca gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa
      241 aagttggcat gcagctctac agcagcgtct gcacccacta ccccgccggg gccccgggtc
      301 ccacggccgc cgcccccgcg ccacccgccg ccgctacccc cttcaaggtc tcgctgcagc
      361 ccccgggagc cgccggcgcc gcgcccgagc ccgagaccgg tgagtgccag cccgccgcgg
      421 ccgccgagca ccgggaagcc gccgctgtcc ccgccgccaa gatgcccgcc ttctcctcct
      481 gcttcgaggt ggtgtccggg gccgccgcgc ccgcctccgc cgccgccggc ccgcccggcg
      541 cgtcctgcaa gccgccgctg ccgccgcact acacgtccac cgcacagatc accgtgcggg
      601 ccctgnnngc ngacaggctc ctgctgcang gncccgatnc ccgttcccgg cgccgcgggc
      661 tccgccgctg cnncnnncgg ncgctgcctc ctgctcgccc cng
//
