LOCUS       sequence-283545-        2303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1427
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaagt cggcgaggac agcggcgccc gcgccctgct cgcgctgcgc
       61 tcggcgccct gcagcccagt gctgtgcgcc gcagccgccg ccgccgcctt ccccgcggcc
      121 gcacccccgc cggcccccgc gcagccccag cctccgcccg ggccgccgcc gccgccgcca
      181 ccgccgctgc ctccgggcgc gatcgcgggc gcgggctcct ccgggggctc ctccggggta
      241 tccggggact ccgcggtcgc gggcgcggcg ccggccctgg tggccgcggc ggccgcctcg
      301 gtacggcaga gcccggggcc ggcgctggcg cggctggagg gccgcgagtt cgagttcctc
      361 atgcgccagc ccagcgtcac catcggccgc aactcgtcgc agggctcggt ggacttgagc
      421 atgggcctgt ccagcttcat ctcgcggcgc cacctgcagc tcagcttcca ggagccgcac
      481 ttctacctgc gctgcctcgg caagaacggc gtcttcgtgg acggggcctt ccagagacgc
      541 ggcgcgcccg ccctgcagct gcccaagcag tgtaccttcc ggtttcccag cacggccatc
      601 aagatccagt tcacgtcgct ctatcacaaa gaagaggccc cagcctcccc gctgcggcca
      661 ctgtaccccc agatctcccc tctgaagatc cacatcccgg agccggacct ccggagcatg
      721 gtcagccccg tcccctcccc gacgggcacc atcagtgtcc ccaactcctg cccagccagt
      781 ccacgcggtg ccggctcctc cagttaccgc tttgtgcaga acgtgacctc ggacctgcag
      841 ctggcagcag agtttgcagc aaaggccgcg tcggagcagc aggcagacac gtctggagga
      901 gacagcccca aggatgagtc aaagccgccg ttctcctacg cgcagctgat cgtgcaggcc
      961 atctcctccg cccaggaccg gcagctgacc ctgagcggga tctacgccca catcaccaag
     1021 cattacccct actaccggac ggccgacaaa ggctggcaga attctatccg gcacaacctc
     1081 tctttgaacc gttactttat caaagtccca cgttcccagg aggagcctgg gaaggggtcc
     1141 ttttggcgaa tagaccctgc ctctgaagcc aagctcgtgg aacaggcatt ccggaaacgg
     1201 aggcagaggg gtgtctcctg cttccgcacc cccttcgggc ctctgtcctc aaggagcgct
     1261 ccagcttcgc ccacacaccc cgggctgatg tcccctcgct ccggcggcct gcagacccca
     1321 gagtgcctgt ctcgggaggg ctcccccatt ccacacgacc ctgagtttgg gtccaagtta
     1381 gcttctgtcc cagagtaccg gtattcccaa agcgcacccg gctcccccgt cagcgcccag
     1441 ccagtgatca tggccgtgcc tccccgaccg tccagcctcg tggccaagcc cgtggcctac
     1501 atgcccgcct ccatcgtaac ctcacagcag cccgcgggcc acgccatcca cgtcgtgcag
     1561 caggccccca ccgtcaccat ggtcagggtg gtcaccacat ctgccaactc ggccaacgga
     1621 tacatcctca ccagccaggg cgcggcgggg ggctcccatg atgcggcggg cgcagccgtg
     1681 ctggacctgg gcagcgaggc cagaggcctg gaggagaaac ccaccattgc gtttgccaca
     1741 atccccgcgg ctggtggagt catccagacg gtggccagcc agatggcccc cggggtcccc
     1801 ggacacacgg tcaccatcct gcagcccgcc acacccgtga ccctcgggca gcaccacctt
     1861 ccagtccggg ccgtgaccca gaacggaaag catgcggttc ccacgaacag tttagccggc
     1921 aacgcttacg ccctcaccag ccctttgcag ctccttgcga cccaagcgag ttcatccgcg
     1981 ccggtggtgg tcacccgggt gtgcgaggtg gggcccaagg agccagcagc agccgtcgcg
     2041 gccacggcca ccaccacccc agccactgcc accaccgcct ctgcctccgc ctcttccact
     2101 ggagagcccg aggtcaaaag gtcccgggtg gaggagccca gtggtgctgt aaccacaccg
     2161 gctggagtga tcgcagctgc cggcccccag gggccaggca ccggggagta gtaatgaact
     2221 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     2281 tgatgactcc tgttggtcta cgc
//
