LOCUS       sequence-283557-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1450
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..291
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHQHH"
     CDS             268..294
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHQHHH"
     CDS             271..297
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHQHHHH"
ORIGIN
        1 accgccacca tgatgaccat gactacgatg gctgacggct tggaaggcca ggactcgtcc
       61 aaatccgcct tcatggagtt cgggcagcag cagcagcagc agcagcaaca gcagcagcag
      121 cagcagcagc aacagcaaca gccgccgccg ccgccgccgc cgccgccgca gccgcactcg
      181 cagcagagct ccccggccat ggcaggcgcg cactaccctc tgcactgcct gcactcggcg
      241 gcggcggcgg cagcggccgg ctcgcaccac caccaccacc accagcacca ccaccacggc
      301 tcgccctacg cgtcgggcgg agggaactcc tacaaccacc gctcgctcgc cgcctacccc
      361 tacatgagcc actcgcagca cagcccttac ctccagtcct accacaacag cagcgcagcc
      421 gcccagacgc gaggggacga cacagatcaa caaaaaacta cagtgattga aaacggggaa
      481 atcaggttca atggaaaagg gaaaaagatt cggaagcctc ggaccattta ttccagcctg
      541 cagctccagg ctttaaacca tcgctttcag cagacacagt atctggccct tccagagaga
      601 gccgaactgg cagcttcctt aggactgaca caaacacagg tgaagatatg gtttcagaac
      661 aaacgctcta agtttaagaa actgctgaag cagggcagta atcctcatga gagcgacccc
      721 ctccagggct cggcggccct gtcgccacgc tcgccagcgc tgcctccagt ctgggacgtt
      781 tctgcctcgg ccaagggtgt cagtatgccc cccaacagct acatgcctgg ctattctcac
      841 tggtactcct ctccacacca ggacacgatg cagagaccac agatgatgta gtaatgaact
      901 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
      961 tgctctttgg agacacgctc aat
//
