LOCUS       sequence-283569-        1622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1471
FEATURES             Location/Qualifiers
     source          1..1622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgtccg cctgtacccc tcggcgcccg cggtgggcgc gcggcccggg
       61 gccgagccgg ccggcctggc gcacctggac tattaccacg gcggcaagtt tgatggtgac
      121 agtgcctacg tggggatgag tgacggaaac ccagagctcc tgtcaaccag ccagacctac
      181 aacggccaga gcgagaacaa cgaagactat gagatccccc cgataacacc tcccaacctc
      241 ccggagccat ccctcctgca cctgggggac cacgaagcca gctaccactc gctgtgccac
      301 ggcctcaccc ccaacggtct gctccctgcc tactcctatc aggccatgga cctcccagcc
      361 atcatggtgt ccaacatgct agcacaggac agccacctgc tgtcgggcca gctgcccacg
      421 atccaggaga tggtccactc ggaagtggct gcctatgact cgggccggcc cgggcccctg
      481 ctgggtcgcc cggcaatgct ggccagccac atgagtgccc tcagccagtc ccagctcatc
      541 tcgcagatgg gcatccggag cagcatcgcc cacagctccc catcaccgcc ggggagcaag
      601 tcagcgaccc cctctccctc cagctccact caggaagagg agtcggaagt gcatttcaag
      661 atctcgggag aaaagagacc ttcagccgac ccaggaaaaa aggccaagaa cccgaagaag
      721 aagaaaaaga aggaccccaa tgagccgcag aagcctgtgt cggcctacgc actcttcttc
      781 agagacactc aggccgccat caagggtcag aaccccagtg ccactttcgg tgacgtgtcc
      841 aaaatcgtgg cctccatgtg ggacagcctg ggagaggaac agaagcaggc ctacaagagg
      901 aagacagaag cagcaaagaa ggaatatctg aaggccctgg cagcctaccg ggctagcctc
      961 gtctccaaga gctccccaga tcaaggtgag accaagagca ctcaggcaaa cccaccagcc
     1021 aaaatgctcc cacccaagca gcccatgtat gccatgccag gcctggcctc cttcctgacg
     1081 ccgtcggacc tgcaggcctt ccgcagtggg gcctcccctg ccagcctcgc ccggacgctg
     1141 ggctccaagt ctctgctgcc aggcctcagt gcgtccccgc cgccgccacc ctccttcccg
     1201 ctcagcccca cactgcacca gcagctgtca ctgccccctc acgcccaggg cgccctcctc
     1261 agtccacctg ttagcatgtc cccagccccc cagccccctg tcctgcccac ccccatggca
     1321 ctccaggtgc agctggcgat gagcccctca cctccagggc cacaggactt cccgcacatc
     1381 tctgagttcc ccagcagctc gggatcctgc tcacctggcc catccaaccc caccagcagc
     1441 ggggactggg acagcagcta ccccagtggg gagtgtggca tcagcacctg cagcctgctc
     1501 cccagggaca aatcgctcta cctcacctag taatgaacta gtccgtaact tgaaagtatt
     1561 tcgatttctt ggctttatat atcttgtgga aaggacgacg agagcctgta gacggagcac
     1621 tt
//
