LOCUS       sequence-283572-        2084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1475
FEATURES             Location/Qualifiers
     source          1..2084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtggt gccagctgag gtgaatagtt tgcttccaga ggaaataatg
       61 gacactggta taactttagt ggatgatgat agtattgagg ctgttattgt ttcatcccca
      121 attcccatgg agacagaact ggaagaaatt gtcaacataa attctactgg tgactctaca
      181 gccacgccca tttccacgga accaatcaca gtgtacagta accacactaa ccaagttgca
      241 gtgaatacca caattactaa agcagattct aataccacag tgaaaccagc ttttccaagt
      301 ggccttcaaa aacttggtgc tcagactcct gtgactatat cagccaatca gattatttta
      361 aacaaagtat cacagacatc tgatcttaaa cttggcaatc agacccttaa accagatgga
      421 cagaagttaa ttttaacaac tttgggcaag tctggttcac caattgtttt agcactaccc
      481 catagccaac taccccaggc tcagaaagtt acaactcagg cccagtcagg agatgctaag
      541 ttaccaccgc agcaaattaa agtagttacc attggaggga ggccagaggt gaaacctgtc
      601 attggtgtct cagcattgac cccaggaagt caactgatta atactacaac tcagccctct
      661 gtgttacaga cccaacagtt aaaaacagta caggcagtga aatcaactgt gcagaccatc
      721 actgttggag gagtgagcac atcacagttt aagacaatta ttcctctggc aactgctccc
      781 aatgtccagc agattcaagt gcctggaagc aagtttcatt atgtccgact tgttactgcc
      841 acatcagcca gtagctcaac ccagccagtt agtcagaatc ccagtacaaa cactcagcct
      901 cttcagcaag caaagccagt ggttgttaat acaaccccag tgcggatgtc agttccaatt
      961 gtctcagctc aggctgtcaa acaagttgtt ccaaaaccaa tcaatccaac ttcacaaata
     1021 gtaactacta gccagccaca gcaacggctt atcatgcctg ccacaccact gccacagatc
     1081 cagcccaacc tcactaacct gccaccaggc actgtcctgg caccagctcc gggaacaggg
     1141 aatgtgggtt atgcagtgct tccagctcag tatgttactc agctacagca gtcttcatat
     1201 gtatcaatag caagcaactc tacctttact ggaacatctg gtatccagac ccaggcacgg
     1261 cttccattca atggcataat cccatcagag tcggccagtc ggccccgaaa gccctgtaat
     1321 tgtacaaaat cactgtgttt gaaattgtat tgtgattgct ttgcaaatgg tgaattttgc
     1381 aacaactgca attgtactaa ttgttacaac aatttggaac atgaaaatga aaggcaaaaa
     1441 gcaataaagg catgccttga cagaaatcca gaagccttta agcctaagat agggaaagga
     1501 aaggagggag aatctgatcg acgtcatagc aaagggtgta attgcaaacg atcaggatgt
     1561 cttaaaaact actgtgaatg ctatgaggca aaaataatgt gttcctcaat atgcaaatgt
     1621 attggctgta agaattttga agaaagcccg gaaaggaaga cattgatgca tttggcagat
     1681 gcagctgaag taagggtaca gcaacaaaca gcagccaaga cgaagttatc ctctcaaatt
     1741 tcagacttgc ttactaggcc aacaccagct ttaaatagtg gaggcggaaa attgccattt
     1801 acatttgtaa ctaaggaagt agctgaagcc acatgtaatt gcctccttgc ccaggcagag
     1861 caggcagaca agaagggaaa atcaaaggca gcagcggaac ggatgatact tgaggaattc
     1921 ggacgatgtt tgatgagtgt catcaactct gcaggaaagg caaaaagtga cccttgtgcc
     1981 atgaattgct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2041 atatcttgtg gaaaggacga ttactagtgc gcagccatct aaac
//
