LOCUS       sequence-283578-        2363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1508
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2020..2031)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgggggaaca cagtccagac aacaacatca tctactttga ggcagaggaa
       61 gatgagctga cccccgatga taaaatgctc aggtttgtgg ataaaaacgg actggtgcct
      121 tcctcatctg gaactgttta tgataggacc actgttctta ttgagcagga ccctggcact
      181 ttggaggatg aagatgacga cggacagtgc ggagaacact tgccttttct agtagggggt
      241 gaagagggct ttcacctgat agatcatgaa gcaatgtccc agggttatgt gcagcacatt
      301 atctcaccag atcagattca tttgacaata aaccctggtt ccacacccat gccaagaaat
      361 attgaaggtg caaccctcac tctgcagtcg gaatgtccgg aaacaaaacg taaagaagta
      421 aagcggtacc aatgtacctt tgagggctgt ccccgcacct acagcacagc aggcaacctg
      481 cgaacccacc agaagactca ccgaggagag tacacctttg tctgtaatca ggagggctgt
      541 ggcaaagcct tccttacctc ttacagcctc aggatccacg tgcgagtgca cacgaaggag
      601 aagccatttg agtgtgacgt gcagggctgt gagaaggcat tcaacacact gtacaggctg
      661 aaagcacatc agaggcttca cacagggaaa acgtttaact gtgaatctga aggctgcagc
      721 aaatacttca ccacactcag tgatctgagg aagcacattc gaactcatac aggggaaaag
      781 ccatttcggt gcgatcacga tggctgtgga aaagcatttg cagcaagcca ccaccttaaa
      841 actcacgttc gtacacatac tggtgaaaga cccttcttct gccccagtaa tggctgtgag
      901 aaaacattca gcactcaata cagtctcaaa agtcacatga aaggtcatga taacaaagga
      961 cactcataca atgcacttcc acaacacaat ggatcagagg atacaaatca ctcactttgt
     1021 ctaagtgact tgagccttct gtccacagat tctgaattgc gagaaaattc cagtacgacc
     1081 cagggccagg acctcagcac aatttcacca gcaatcatct ttgaatcaat gttccagaat
     1141 tcagatgata cggcaattca ggaagatcct caacagacag cttccttgac tgaaagtttt
     1201 aatggtgatg cagagtcagt cagtgatgtt ccgccatcca caggaaattc agcatcttta
     1261 tctcttccac ttgtactgca acctggcctc tccgagccac cccagcctct actacctgcc
     1321 tcagctccgt ctgctcctcc gcctgctccc tccctaggac ctggctccca gcaagctgca
     1381 tttggcaacc cccctgctct cttacaacct ccagaagtgc ctgttcccca cagcacacag
     1441 tttgctgcta atcatcaaga gtttcttccg cacccccagg caccgcagcc cattgtacca
     1501 ggactttctg ttgttgctgg ggcttctgca tcagcagcgg cagtggcatc agctgtggca
     1561 gcaccagccc caccacaaag tactactgag cccctgccag ccatggtcca gactctgccc
     1621 ctgggtgcca actctgtcct aactaataat cccacaataa ccatcacccc aactcccaac
     1681 acagctatcc tgcagtccag cctagtcatg ggagaacaga acttacaatg gatattaaat
     1741 ggtgccacca gttctccaca aaaccaagaa caaattcagc aagcatctaa agttgagaag
     1801 gtgtttttta ccactgcagt accagtagcc agtagcccag ggagctctgt ccagcagatt
     1861 ggcctcagtg ttcctgtgat catcatcaaa caagaagagg catgtcagtg tcagtgtgca
     1921 tgccgggact ctgcaaagga gcgggcatcc agcaggagaa agggctgctc ctccccaccc
     1981 cctccagagc cgagccccca ggctcctgat gggcccagcc tgcagctccc agcgcagact
     2041 ttctcttcag cccctgttcc cgggtcatca tcctctacct tgccctcctc ctgtgagcaa
     2101 agccgacaag cagagactcc ttcagaccct cagacagaaa cattaagtgc catggatgtg
     2161 tcagagtttc tatccctcca gagcctggac accccgtcca atctgattcc cattgaagca
     2221 ctactgcagg gggaggagga gatgggcctc accagcagct tctccaagta gtaatgaact
     2281 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2341 ttgagagctg atttgctgtt cgt
//
