LOCUS       sequence-28358-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-PSMD14
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..70
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     86..102
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      131..140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             140..163
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             176..202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    218..242
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acnccctaag cctccgcctc ctcttcctcc atccgnnnnn nntctccccc ttgaacctcc
       61 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
      121 aattagatct gccaccatgg actacaagga tgacgatgac aagctcgatg gaggataccc
      181 atacgatgtt ccagattacg ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg
      241 catggacaga cttcttagac ttggaggagg tatgcctgga ctgggccagg ggccacctac
      301 agatgctcct gcngtggaca cagcagaaca agtctatatc tcttccctgg cactgttaaa
      361 aatgttaaaa catggccgtg ctggagttcc aatggaagtt atgggtttga tgcttggaga
      421 atttgttgat gattataccg tcagagtgat tgatgtgttt gctatgccac antcaggaac
      481 aggtgtcagt gtggaggcag ttgatccagt gttccaagct aaaatgttgn atatgttgaa
      541 gcagacagga aggccggaga tggttgttgg ttggtatcac agtcaccctg gctttggntg
      601 ttggctttct ggtgtggata tcnnnnctca gcagagcttt gaagccttgt cggagagagc
      661 tgtggcagtg gttgtggatc ccnttcanag tgtaaaagga aaggttgnta ttgatgcctt
      721 canattgatc aatgntaata tgatggnctt aggacntgaa ccaagacaaa cnacttcgaa
      781 tctgggtcac ttnaacaagc catct
//
