LOCUS       sequence-283581-        1712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1516
FEATURES             Location/Qualifiers
     source          1..1712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggccatt gcaatttaga gacgtggcca tagaattctc tctggaggag
       61 tggcattgcc tggacactgc acagcggaat ttatatagga atgtgatgtt agagaactac
      121 agaaacctgg tcttccttgg tattgttgtc tctaagccag acctcatcac ctgtctggag
      181 caaggaaaaa aacctttgac catgaagaaa catgagatgg tagccaaccc ctcagttacg
      241 tgttctcatt ttgcccgaga tctttggcca gagcagagca taaaagattc tttccaaaaa
      301 gtgacactga gaagatatga aaactatgga catgacaatt tacagtttaa aaagggctgt
      361 gaaagtgtag atgagtgtaa ggtgcacaaa agaggttata atggacttaa ccaatatttg
      421 acaactactc aaagcaaaat atttcagtgt gataaatatg tgaaagtcat tcataaattt
      481 tcaaattcaa acagacataa gataagacat actggaaaaa aacctttcaa atgtatagaa
      541 tgtggcaaag cttttaacca gtcttcaacc cttactacac ataagaaaat tcatactggg
      601 gagaaaccct tcaaatgtga agaatgtggc aaagccttca actggtcctc acaccttact
      661 acacataaga gaattcatac tggagagaaa cggtacaaat gtgaagactg tggcaaagcc
      721 tttagccggt tttcatacct tactgcacat aagataattc atagtggaga gaaaccctac
      781 aaatgtgaag aatgtggcaa ggcctttaag cgctcctcta accttactac acataagata
      841 attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaggcctt taagcgctcc
      901 tctatcctta ctgcgcataa gataattcat agcggagaga aaccctacaa atgtgaagaa
      961 tgtggcaaag cctttaagca cccctctgtc cttactacac ataaaagaat tcatactgga
     1021 gagaaaccct acaaatgtga agaatgtggc agagccttta agtacttctc atcccttact
     1081 acacacaaga taattcatag tggagagaaa ccctacaaat gtgaagaatg tggcaaagcc
     1141 ttcaactggt cctcacacct tactacacat aagagaattc atactggaga gaaaccctac
     1201 aaatgtgaag aatgtggcga agcctttaag tactcctctt cccttactac acataagata
     1261 atccatactg gacagcaacc cttcaagtgt gaagaatgtg gcaaggcctt taagtgcttc
     1321 tctatcctta ctacacataa gagaattcat actggagaga aaccctacaa atgtgaagaa
     1381 tgtggcaaag ccttcaactc gtcctcacac cttactgcac ataagagaat tcatactgga
     1441 gagaaaccct acaaatgtga acgatgtggc aaggctttta agcgctcctt tatccttact
     1501 agacataaga gaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaaggc
     1561 tttaagtgcc cctctaccct tactacacat aaggtaattc atactggaga gaaactatag
     1621 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1681 aaggacgatt ggtttcgtta ggtactgtgc ac
//
