LOCUS       sequence-283599-        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1542
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagacaga tttggctgaa atgcctgaga aaggagttct gtcttcccag
       61 gattctcccc atttccaaga gaagagcaca gaagagggag aagtggctgc tctgcgcctc
      121 acggccagat cccaggcggc ggcggcggcg gcggcgcccg ggagccggag ccttcgcggc
      181 gtccacgtcc ctcccccgct gcaccccgcc ccggcgcgag aggagatcaa atccacttgc
      241 tccttgaagg catgcttttc actttcatta actcttacct attacagaac tgcctttttg
      301 ttgagcacag aaaacgaagg aaatttgcat ttccaatgtc cttcagacgt ggagactaga
      361 ccacagagca aggattcaac ttcagtgcaa gatttttcca aagcagaatc atgcaaagtt
      421 gcaataatag acagactgac acggaatagt gtctatgact ctaacttgga agcagccctt
      481 gaatgtgaaa attggttaga gaagcagcaa ggaaatcagg agagacattt gagagaaatg
      541 ttcactcaca tgaattcact ctctgaggaa acagaccatg agcatgatgt atactggaaa
      601 agcttcaatc agaaatctgt ccttatcact gaagacagag ttcccaaagg atcttatgcc
      661 ttccatacac ttgaaaaaag cttgaaacaa aaatcaaact taatgaaaaa gcagagaact
      721 tataaagaga aaaaacctca taaatgtaat gattgtggtg aactcttcac ctgccattca
      781 gtgcatattc aacaccagag agtccatact ggagagaaac cctatacctg caatgaatgt
      841 gggaaatctt ttagccacag agctaattta actaaacacc agagaactca tactagaatt
      901 ctctttgaat gccgtgaatg taagaaaacc ttcacagaaa gctcatccct tgcaacacat
      961 cagagaattc acgttggaga gagaccttat gaatgcaatg aatgtgggaa aggttttaat
     1021 cgaagtacac atcttgtgca gcatcagttg attcatactg gagtgaggcc ttatgaatgt
     1081 aatgaatgtg ataaagcttt tattcattca tcagcactca ttaaacatca aagaactcat
     1141 actggagaga aaccttataa atgtcaagaa tgtgggaaag cctttagcca ttgctcatcc
     1201 ctaactaagc atcagagagt tcatactgga gaaaagccat atgaatgcag tgaatgtgga
     1261 aaaactttta gtcagagcac acatcttgtt caacatcaga gaattcacac tggagagaaa
     1321 ccctatgagt gtagtgaatg tgggaaaacc ttcagccaga gctccaattt tgctaaacat
     1381 caaagaattc atattggaaa gaaaccgtac aaatgtagtg agtgtggaaa agccttcatt
     1441 cattcatcag ctcttattca acatcagaga actcataccg gagagaaacc ctttagatgt
     1501 aatgagtgtg ggaaaagctt taagtgcagt tcatctctca tcagacatca aagagttcac
     1561 actgaagagc aaccctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1621 ctttatatat cttgtggaaa ggacgaacgg ttctctaggc tgcaagaccc
//
