LOCUS       sequence-28361-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-STAMBP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..28
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     44..60
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             134..160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    176..200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
       61 tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg acgatgacaa
      121 gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt atcaaacaag
      181 tttgtacaaa aaagttggca tgtctgacca cggagatgtg agcctcccgc ccgaagaccg
      241 ggtgagggct ctctcccagc tgggtagtgc ggtagaggtg aatgaagaca ttccaccccg
      301 tcggtacttc cgctctggag ttgagattat ccgaatggca tccatttact ctgaggaagg
      361 caacattgaa catgccttca tcctctataa caagtatatc acgctcttta ttgagaaact
      421 accaaaacat cgagattaca aatctgctgt cattcctgaa aagaaagaca cagtaaagaa
      481 attaaaggag attgcatttc ccaaagcaga agagctgaag gcagagctgt taaaacgata
      541 taccaaagaa tatacagaat ataatgaaga aaagaagaag gaagcagagg aattggnccg
      601 gaacatggcc atccagcaag agctggaaaa ggaaaaacag anggtagcac aacagaagca
      661 gcagcaattg naacanganc agttccatgc cttcnaggag atgatccgga nnnnngagct
      721 agaaaaanag cgactgaaaa ttgtacagga ntttggnaag gnagaccctg gnctaggtgg
      781 nccgctagtg cctgantngn anaagccctc cttagatgng ttccccacct
//
