LOCUS       sequence-283614-        5813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1606
FEATURES             Location/Qualifiers
     source          1..5813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1030..1041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggaggcaaa cgaccatttt aactttactg gccttccccc tgcacctgct
       61 gcctcaggac tgaaaccctc tccttcctca ggggagggcc tctacactaa cgggtctccc
      121 atgaacttcc cccagcaagg gaaaagtttg aatggggatg tgaatgttaa tggcttatct
      181 actgtatctc acactactac ttcagggatt ttgaactctg ctccccactc ctccagcacc
      241 tcacacctcc atcaccccag cgtggcctac gactgtctct ggaactactc acagtaccca
      301 tctgccaatc ctggcagcaa cctcaaggac ccaccccttc tctcccagtt ctcgggggga
      361 caatacccac tcaacggcat ccttgggggc agccggcaac cttcatcccc aagtcataac
      421 actaaccttc gggctgggag ccaagagttc tgggccaacg gtacccagag tcccatgggg
      481 cttaactttg attcacaaga actgtatgat tcctttcctg accagaattt tgaggtgatg
      541 cccaatggac cccctagttt tttcacctcc ccacagactt ctcctatgtt gggatctagc
      601 attcaaacct ttgcaccctc ccaggaggta ggcagtggta tccatcctga tgaggcagca
      661 gaaaaggaga tgacttcagt tgtggcagag aatggcactg gcttggtagg cagcttggag
      721 ctggaagaag agcagccaga actgaagatg tgtggctaca atggctctgt cccttctgtg
      781 gaatcgttac accaagaggt ctcagtcctg gtccctgacc ccacagtgag ctgtttagat
      841 gatccttcac atcttcctga tcaactggaa gacactccaa tcctcagtga agactctctg
      901 gagcccttca actctctggc accagagcca gtgagtggag gactatatgg tattgatgac
      961 acggagctga tgggtgcaga ggacaagctg cctcttgagg acagccctgt gatttctgcc
     1021 cttgattgcc cttccctcaa taatgctact gccttcagtc tcctggcaga tgatagtcaa
     1081 acatcaacct ctatctttgc cagtcccacc tctccacctg tcctagggga gtctgtcctg
     1141 caagataaca gctttgacct gaataatggt agtgacgctg aacaggaaga aatggaaact
     1201 caatcttcag acttcccacc atccctgacc cagccagctc ctgatcagtc atccactatt
     1261 cagctacatc cagcaacctc accagcagtc tcgccaacaa cctccccagc agtctcccta
     1321 gtggtttctc cagcagcctc cccagaaatc tctccagaag tttgtcccgc agcttctaca
     1381 gttgtctctc cagcagtctt ctcagtggtc tctccagctt cctcagcagt cctcccagca
     1441 gtctccttag aagtcccgtt gacggcttca gtgacatccc caaaagcctc tcccgtaact
     1501 tccccagcag ctgcctttcc aacagcctcc ccagcaaata aggatgtcag cagctttcta
     1561 gaaaccactg ctgacgtgga agagatcact ggagaaggac tcactgcttc tggtagtggt
     1621 gatgtcatga ggagacgtat tgctacccca gaagaagttc gtcttcccct ccaacatggg
     1681 tggcggagag aggtgcgcat caagaagggc agccaccgat ggcaggggga gacctggtat
     1741 tatggcccct gtgggaagag gatgaagcaa tttccagaag tgatcaagta cctgagccgc
     1801 aacgtggtac acagtgtccg ccgagagcac ttcagcttca gtccccgtat gcctgttgga
     1861 gatttctttg aagaaagaga cacgccagag ggcttgcagt gggtgcagct ctcagcagag
     1921 gagatcccgt cgaggattca ggcaattact ggcaaacggg gtcgacctcg aaacactgag
     1981 aaggctaaga ctaaggaagt ccccaaggtg aaacggggtc gaggtcggcc acctaaggtc
     2041 aaaatcactg agctattgaa caagacagac aaccgccccc taaagaaact ggaggcccaa
     2101 gaaacattga atgaggagga taaagcaaag attgctaaaa gcaagaagaa gatgaggcag
     2161 aaggttcaac ggggagagtg tcagactact atccaagggc aggccagaaa taagcggaaa
     2221 caagagacca agagcttaaa gcagaaggaa gctaagaaga aatccaaggc tgagaaagaa
     2281 aaaggaaaga caaagcagga aaaactgaag gaaaaagtca agagggaaaa gaaggagaag
     2341 gtaaaaatga aggaaaagga ggaggtgacc aaagccaagc cagcctgtaa agcagataag
     2401 accctggcca cacagaggcg cttggaggaa cggcagaggc agcagatgat cttggaggaa
     2461 atgaagaagc cgacagagga tatgtgtctg actgaccacc agcccctgcc tgacttctca
     2521 cgagtccctg gtctgacatt gcccagtgga gccttctcag actgcttgac cattgtggag
     2581 ttcctgcata gctttggcaa ggtgctgggc tttgatcctg ccaaagatgt gcctagcctg
     2641 ggggtcctgc aggagggact cctgtgtcaa ggtgacagct tgggtgaggt gcaagacctg
     2701 ctggtcaggc tgctgaaggc tgcactccat gatcctggct ttccctccta ctgtcagtcc
     2761 ctaaagatct tgggggagaa ggtgtctgag atcccactga caagagacaa tgtgtcagag
     2821 atcctgcgct gcttccttat ggcatatgga gtagagccag ccctctgtga ccgcctgcgc
     2881 acccagcctt ttcaggccca gccaccccag cagaaggctg ctgtcctggc cttccttgtg
     2941 catgagctca atggctccac cctcatcatc aatgagattg acaagactct ggagagtatg
     3001 tccagctaca ggaaaaacaa gtggattgtt gaaggccggc tccggaggct gaaaactgtt
     3061 ctggccaagc gaactgggcg gtctgaagta gagatggaag ggccagagga atgcctggga
     3121 cggaggcgca gttctcggat catggaggag accagtggca tggaagaaga ggaagaagag
     3181 gagtctatag cagctgtccc tggccgcagg ggtcgaagag atggagaggt tgatgccaca
     3241 gcatctagca tcccagagct agagcgccag atagaaaaac tcagcaagcg tcagcttttc
     3301 tttcgcaaaa agctgcttca ctcatcccag atgcttcggg cggtctccct gggtcaggac
     3361 cgctacagac gtcgctactg ggtattgccg tatttggctg gtatctttgt agaaggaaca
     3421 gaggggaact tagttcctga ggaggtgata aagaaggaaa ctgactcctt aaaagtggca
     3481 gcccatgcgt cactcaaccc tgccctcttc tctatgaaga tggagttagc tggctccaac
     3541 accactgcca gttctcctgc ccgggcccga ggccgacctc gaaaaactaa gcccgggtct
     3601 atgcaaccta ggcatcttaa gtcccctgtc aggggtcagg attcagaaca gccccaggcc
     3661 cagcttcagc ctgaggctca gcttcatgct cctgcccagc cccagcctca gcttcagctt
     3721 cagcttcagt cccataaggg gttcctggag caagaaggct cccctttgtc actgggtcag
     3781 agccagcatg acctcagcca gtcagccttc ctgtcttggc tgagccagac tcagagccat
     3841 agctccctgt tgagcagctc agtcctcaca cctgatagca gtccgggaaa actagaccca
     3901 gctccatcac aacccccgga ggagccagag cctgatgagg cagaatccag ccctgatcct
     3961 caagcactct ggtttaacat ctcagcccag atgccctgca atgctgcccc cacaccgccc
     4021 cctgcagttt ctgaggacca acccactccc tcccctcagc agcttgcctc ctccaagcca
     4081 atgaatagac ctagtgctgc caacccttgt tctccagtgc agttctcttc cacgcccttg
     4141 gctgggttgg cccctaagag gcgagcagga gaccctggag aaatgccaca gagtcccaca
     4201 gggctgggac agcccaaacg gagagggaga cctcccagta agttcttcaa acagatggaa
     4261 cagcgttacc taacccagct gacagcccag cctgtcccac ctgagatgtg ctcaggctgg
     4321 tggtggatac gagatcctga gatgttggat gccatgctca aggccctaca cccccgaggt
     4381 atccgggaga aggcacttca caaacacctt aacaagcaca gggacttctt gcaggaagtc
     4441 tgcctgcggc cctcagctga ccccatcttt gagcccaggc aactacctgc ctttcaagaa
     4501 gggattatga gctggtcccc caaagagaag acatacgaga cagacctagc agtgcttcaa
     4561 tgggtagagg agctggagca gcgggttatc atgtctgatc tgcagattcg gggctggaca
     4621 tgtcctagcc cagactctac ccgtgaagac ttggcctact gtgagcacct ctccgactcc
     4681 caggaggata tcacctggcg aggtcggggc agggagggac tggcacctca gcgtaaaact
     4741 accaaccctt tggacctggc tgtgatgcgg ctggctgccc tggaacagaa tgtagaacgg
     4801 cggtacctgc gggagcccct ctggccaact catgaggttg tgctggagaa ggccctgctt
     4861 agcacaccta atggtgcccc tgagggcacc actacagaga tatcatatga gatcacccct
     4921 cgcattcgtg tctggcgcca gaccctcgag cggtgccgga gcgcagccca ggtgtgcttg
     4981 tgcctgggcc agctggagag gtccattgcc tgggagaagt ctgtcaacaa agtgacatgt
     5041 ctagtctgcc ggaagggtga caatgatgag tttcttctgc tttgtgatgg gtgtgaccgt
     5101 ggctgccaca tttactgcca tcgtcccaag atggaggctg tcccagaagg agattggttc
     5161 tgtactgtct gtttggctca gcaggtggag ggagaattca ctcagaagcc tggtttccca
     5221 aagcgtggcc agaagcggaa aagtggttat tcgctgaact tctcagaggg tgatggccgc
     5281 cgacgccggg tactgttgag gggccgagaa agcccagcag cagggcctcg gtactcggaa
     5341 gaagggctct ccccctccaa gcggcggcga ctctctatgc ggaaccacca cagtgatctc
     5401 acattttgcg agattatcct gatggagatg gagtcccatg atgcagcctg gcctttccta
     5461 gagcctgtga acccacgttt ggtgagtggg taccggcgca tcatcaaaaa tcctatggat
     5521 ttttccacca tgcgggagcg gctgctcagg ggagggtaca ccagctcaga ggagtttgcg
     5581 gctgatgccc tcctggtatt tgacaactgc cagactttca acgaggatga ctctgaagta
     5641 ggcaaggctg ggcacatcat gcgccgcttc ttcgagagcc gctgggagga gttttatcag
     5701 ggaaaacagg ccaatctgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     5761 tggctttata tatcttgtgg aaaggacgaa tgatgactgt cattccgcga gta
//
