LOCUS       sequence-283620-        2939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1661
FEATURES             Location/Qualifiers
     source          1..2939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgccaaa tcttaaaggc agaccacgca aaaagaaacc atgcccacaa
       61 agaagagatt cattcagtgg tgttaaggat tccaacaaca attccgatgg caaagccgtt
      121 gccaaggtga aatgtgaggc caggtcagcc ttgaccaagc cgaagaataa ccataactgt
      181 aaaaaagtct caaatgaaga aaaaccaaag gttgccattg gtgaagagtg cagggcagat
      241 gaacaagcct tcttggtggc actttataaa tacatgaaag aaaggaaaac gccgatagaa
      301 cgaataccct atttaggttt taaacagatt aacctttgga ctatgtttca agctgctcaa
      361 aaactgggag gatatgaaac aataacagcc cgccgtcagt ggaaacatat ttatgatgaa
      421 ttaggcggta atcctgggag caccagcgct gccacttgta cccgcagaca ttatgaaaga
      481 ttaatcctac catatgaaag atttattaaa ggagaagaag ataagcccct gcctccaatc
      541 aaacctcgga aacaggagaa cagttcacag gaaaatgaga acaaaacaaa agtatctgga
      601 accaaacgca tcaaacatga aatacctaaa agcaagaaag aaaaagaaaa tgccccaaag
      661 ccccaggatg cagcagaggt ttcatcagag caagaaaaag aacaagagac tttaataagc
      721 cagaaaagca tccctgagcc tctcccagca gcagacatga agaaaaaaat agaagggtat
      781 caggaatttt cagcgaagcc cctggcatcc agagtagacc cagagaagga caacgaaaca
      841 gaccaaggtt ccaacagtga gaaggtggca gaggaggcgg gagagaaggg gcccacacct
      901 ccactcccaa gtgctcctct ggccccagaa aaagattcag ccttggtccc tggggccagc
      961 aaacagccac tcacctctcc tagtgccctg gtggactcaa aacaagaatc caaactgtgc
     1021 tgttttacag agagccctga aagtgaaccc caagaagcat ccttccccag cttccccacc
     1081 acacagccac cgctggcaaa ccagaatgag acggaggatg acaaactgcc cgccatggca
     1141 gattacattg ccaactgcac cgtgaaggtg gaccagctgg gcagtgacga catccacaat
     1201 gcgctcaagc agaccccaaa ggtccttgtg gtccagtcgt ttgacatgtt caaagacaaa
     1261 gacctgactg ggcccatgaa cgagaaccat ggacttaatt acacgcccct gctctactct
     1321 aggggcaacc caggcatcat gtccccactg gccaagaaaa agcttttgtc ccaagtgagt
     1381 ggggccagcc tctccagcag ctacccttat ggctccccac cccctttgat cagcaaaaag
     1441 aaactgattg ctagggatga cttgtgttcc agtttgtccc agacccacca tggccaaagc
     1501 actgaccata tggcggtcag ccggccatca gtgattcagc acgtccagag tttcagaagc
     1561 aagccctcgg aagagagaaa gaccatcaat gacatcttta agcatgagaa actgagtcga
     1621 tcagatcccc accgctgcag cttctccaag catcacctta acccccttgc tgactcctac
     1681 gtcctgaagc aagaaattca ggagggcaag gataaactct tagagaaaag ggccctcccc
     1741 cattcccaca tgcctagctt cctggctgac ttctactcgt cccctcatct ccatagcctc
     1801 tacagacaca ccgagcacca tcttcataat gaacagacat ccaaataccc ttccagggac
     1861 atgtacaggg aatcggaaaa cagttctttt ccttcccaca gacaccaaga aaagctccat
     1921 gtaaattatc tcacgtccct gcacctgcaa gacaaaaagt cggcggcagc agaagcccct
     1981 acggatgatc agcctacaga tctgagcctt cccaagaacc cgcacaaacc taccggcaag
     2041 gtcctgggcc tggctcattc caccacaggg ccccaggaga gcaaaggcat ctcccagttc
     2101 caggtcttag gcagccagag tcgagactgt caccccaaag cctgtcgggt atcacccatg
     2161 accatgtcag gccctaaaaa ataccctgaa tcgctttcaa gatcaggaaa acctcaccat
     2221 gtgagactgg agaatttcag gaagatggaa ggcatggtcc acccaatcct gcaccggaaa
     2281 atgagcccgc agaacattgg ggcggcgcgg ccgatcaagc gcagcctgga ggatttggac
     2341 cttgtgattg cagggaaaaa ggcccgggca gtgtctccct tagacccatc caaggaggtc
     2401 tctgggaagg agaaggcctc tgagcaggag agtgaaggca gcaaagcagc gcacggtggg
     2461 cattccgggg gcggatcaga aggccacaag cttcccctct cctcccctat cttcccaggt
     2521 ctgtattccg ggagcctgtg taactcgggc ctcaactcca ggctcccggc tgggtattct
     2581 cattctctgc agtacttgaa aaaccagact gtgctttctc cactcatgca gcccctggct
     2641 ttccactcgc ttgtgatgca aagaggaatt tttacatcac cgacaaattc tcagcagctg
     2701 tacagacact tggctgcggc tacacctgta ggaagttcat atggggacct tttgcataac
     2761 agcatttacc ctttagctgc tataaatcct caagctgcct ttccatcttc ccagctgtca
     2821 tccgtgcacc ccagtacaaa actgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2881 atttcttggc tttatatatc ttgtggaaag gacgatgaaa cctccacggc actccatga
//
