LOCUS       sequence-28363-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-LOC220594
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..77
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     93..109
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctttgtacn ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
       61 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
      121 gcgccggaat tagatctgcc accatggact acaaggatga cgatgacaag ctcgatggag
      181 gatacccata cgatgttcca gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa
      241 aagttggcat gagatatact actgttccaa gtgtaagacc cactgcttta gcaacaaaga
      301 agctggatct ctggaggctt ccacccttcc tgattattca ccttaagcga tttcaatttg
      361 taaatgatca gtggataaaa tcacagaaaa ttgtcaaatt tcctcgggaa agttttgatc
      421 cgagtgcttt tttggtacca cgagacccgg ctctctgcca gcatcaacca ctcacacccc
      481 agggggatga gctctccaag cccaggattc tggcaagaga ggtgaagaaa gtggatgtgc
      541 agagtttggc tggggaagag gacatgctcc tgagcaaaag cccatcctca ctcagcgcta
      601 acatcagcag cagcccaaaa ggttctcctt cttcatcaag aaaaagtgga accagctgtc
      661 cctccagcaa aaacagcagc cctaatagct gcccacggac tttggggagg agcaaaggga
      721 ggctccngct gccccagatt ggcagcaaaa ataaactgtc aagtagtaag aanaacttgg
      781 atgccagcaa aganaatggg gctgggcaga tctgtganct ggctgangnc ttgagctgag
      841 ggcatatgcg ggggggcagc caaccaganc tggncactcc tcnnnacc
//
