LOCUS       sequence-28364-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-UCHL1
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..85
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     101..117
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             155..178
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             191..217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtcagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
       61 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
      121 ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg atgacaagct
      181 cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc aaacaagttt
      241 gtacaaaaaa gttggcatgc agctcaagcc gatggagatc aaccccgaga tgctgaacaa
      301 agtgctgtcc cggctggggg tcgccggcca gtggcgcttc gtggacgtgc tggggctgga
      361 agaggagtct ctgggctcgg tgccagcgcc tgcctgcgcg ctgctgctgc tgtttcccct
      421 cacggcccag catgagaact tcaggaaaaa gcagattgaa gagctgaagg gacaagaagt
      481 tagtcctaaa gtgtacttca tgaagcagac cattgggaat tcctgtggca caatcggact
      541 tattcacgca gtggccaata atcaagacaa actgggattt gaggatggat cagttctgaa
      601 acagtttctt tctgaaacag agaaaatgtc ccctgaagac agagcaaaat gctttgaaaa
      661 gaatgaggcc atacaggcag cccatgatgc cgtggcacag gaagg
//
