LOCUS       sequence-283641-        2030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1719
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggaaga aagaaaaact gctgagttac agaaaaatag aatacaggac
       61 tcagtagtct ttgaggatgt ggctgtggac tttacccagg aggaatgggc tttgctggat
      121 cttgctcaga gaaacctcta cagagatgtg atgctggaaa acttccagaa cctggcctca
      181 ctagggtatc cgctacacac accccatctg atctcccagt gggaacaaga ggaggacctg
      241 cagacagtga agagagaact tatccagggc atctttatgg gagagcaccg ggaaggtttt
      301 gagactcaac ttaaaactaa tgaatcagtt gcttcacaag atatttgtgg agaaaaaata
      361 tccaatgaac agaaaatagt aagattcaaa aggaatgatt cctggttctc cagtttacat
      421 gaaaaccagg agtcctgtgg tattgattat cagaacaaaa gccatgagag acatttgaga
      481 aatcatatgg tagagaacat ctatgaatgt tatgaagaaa atcaagatgg acagactttc
      541 agccaagttc caaatcttga ttcactcaag agaaatactg aagtaaaatc ctgtgaatgc
      601 catgagtgtg gaaaggcctt cgtggatcat tcatccctta agagtcacat caggtctcat
      661 actggaagca aaccctatca gtgcaaggaa tgtgggaagg ccttccattt tcttgcttgt
      721 ttcaagaagc atatgaaaac ccccactgaa gagaagccct atgaatgtaa ggaatgtacc
      781 aaagccttca gctgttcctc attctttagg gcacatatga agattcacat cggaaagaca
      841 aactatgaat gtaaagaatg tgggaaaggt tttagttgtt cctcatcgct cacagaacac
      901 aaaagaattc acagtggaga taagccttat gaatgtaagg aatgtgggaa ggccttcagt
      961 tgttcctcct cactctccaa acacaaaaga attcacagtg gagataagcc atatgaatgt
     1021 aaggaatgtg ggaaggcctt tagctcttcc tctcacctta taatacatat aagaattcac
     1081 actggagaga agccttatga atgtaaagag tgtgggaagg ccttcagcga gtcctcaaaa
     1141 ctcactgtac atgggaggac acatactgga gagaaaccct ataaatgtaa ggaatgtggg
     1201 aaagcctaca attgtccctc ctccttaagt atccatatga gaaaacacac tggagaaaaa
     1261 ccgtatgaat gtctggaatg tggaaaggcc ttctaccttc ccacttccct taatacacat
     1321 gtgaaaaatc aaagtcgaga gaaaccctat gaatgtaagg aatgtgggaa ggcctttagt
     1381 tgtccctcgt cctttagagc acatgtgaga gatcacactg gaaagataca gtatgaatgt
     1441 aaggagtgtg ggaagacctt tagtcgttcc tcatccctca ccgaacacct aagaactcac
     1501 agcggagaga agccgtatga atgtaaagaa tgtgggaaag cctttattag ttcctcccac
     1561 cttacagtac atatcagaac tcacactgga gagaaaccct atgaatgtaa gaaatgtggg
     1621 aaagccttta tttatccctc agcccttagg atccacatga gaacgcacac tggggaaaaa
     1681 ccctatgaat gtaaggaatg tgggaaagca tttcgtcatt cttcatacct tactgtacat
     1741 gcaagaatgc acactggaga gaaacccttt gaatgtctgg aatgtggaaa agctttcagt
     1801 tgtccctcat cctttcgaag gcatgtgaga agccacactg gagagaaacc ctatgaatgt
     1861 aaggaatgtg ggaaagcctt tgtttgtcct gcctactttc gaaggcatgt gaaaactcac
     1921 actagagaaa acatatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1981 ctttatatat cttgtggaaa ggacgaatct tacaccctag cagaggtcgt
//
