LOCUS       sequence-283644-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1721
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaccc gcaggctggc tccgcggccg gggactggga gatcgatgtc
       61 gagagcctgg agctggaaga ggacgtctgc ggggcgccgc ggtccacgcc ccccgggccc
      121 agcccgccgc cggcggacgg ggactgcgag gacgacgaag atgacgacgg ggtggacgaa
      181 gacgcggaag aagagggcga cggcgaggag gcaggcgcgt cccccgggat gcccggccag
      241 ccggagcagc gggggggacc gcagccgagg ccgccgctcg cgcctcaggc ctcacccgcc
      301 ggcaccggtc cccgagagcg ctgcactccc gcgggcggcg gcgcggagcc gcgcaagctg
      361 agccgcacgc ccaagtgcgc gcgctgccgc aaccacggcg tggtgtcctg cctgaagggc
      421 cacaagcgct tctgtcgctg gcgcgactgc cagtgcgcca actgcctgct ggtggtggag
      481 cggcagcgcg tcatggccgc ccaggtggcg ctccggaggc agcaggccac cgaggacaag
      541 aaggggcttt ccgggaaaca gaataatttc gagcgcaaag ctgtgtacca gaggcaagtc
      601 agagccccca gtttgctggc caaaagcatt ttagaagttc tccttggatt attctacagc
      661 tattatgtgt acataatgaa ccatctttag taatgaacta gtccgtaact tgaaagtatt
      721 tcgatttctt ggctttatat atcttgtgga aaggacgacc aagaggcaca gactcgcgtg
      781 tg
//
