LOCUS       sequence-283647-        2897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1736
FEATURES             Location/Qualifiers
     source          1..2897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagat caaggagaaa atctgcgact atctcttcaa tgtgtctgac
       61 tcctctgccc tgaatttggc taaaaatatt ggccttacca aggcccgaga tataaatgct
      121 gtgctaattg acatggaaag gcagggggat gtctatagac aagggacaac ccctcccata
      181 tggcatttga cagacaagaa gcgagagagg atgcaaatca agagaaatac gaacagtgtt
      241 cctgaaaccg ctccagctgc aatccctgag accaaaagaa acgcagagtt cctcacctgt
      301 aatataccca catcaaatgc ctcaaataac atggtaacca cagaaaaagt ggagaatggg
      361 caggaacctg tcataaagtt agaaaacagg caagaggcca gaccagaacc agcaagactg
      421 aaaccacctg ttcattacaa tggcccctca aaagcagggt atgttgactt tgaaaatggc
      481 cagtgggcca cagatgacat cccagatgac ttgaatagta tccgcgcagc accaggtgag
      541 tttcgagcca tcatggagat gccctccttc tacagtcatg gcttgccacg gtgttcaccc
      601 tacaagaaac tgacagagtg ccagctgaag aaccccatca gcgggctgtt agaatatgcc
      661 cagttcgcta gtcaaacctg tgagttcaac atgatagagc agagtggacc accccatgaa
      721 cctcgattta aattccaggt tgtcatcaat ggccgagagt ttcccccagc tgaagctgga
      781 agcaagaaag tggccaagca ggatgcagct atgaaagcca tgacaattct gctagaggaa
      841 gccaaagcca aggacagtgg aaaatcagaa gaatcatccc actattccac agagaaagaa
      901 tcagagaaga ctgcagagtc ccagaccccc accccttcag ccacatcctt cttttctggg
      961 aagagccccg tcaccacact gcttgagtgt atgcacaaat tggggaactc ctgcgaattc
     1021 cgtctcctgt ccaaagaagg ccctgcccat gaacccaagt tccaatactg tgttgcagtg
     1081 ggagcccaaa ctttccccag tgtgagtgct cccagcaaga aagtggcaaa gcagatggcc
     1141 gcagaggaag ccatgaaggc cctgcatggg gaggcgacca actccatggc ttctgataac
     1201 cagcctgaag gtatgatctc agagtcactt gataacttgg aatccatgat gcccaacaag
     1261 gtcaggaaga ttggcgagct cgtgagatac ctgaacacca accctgtggg tggccttttg
     1321 gagtacgccc gctcccatgg ctttgctgct gaattcaagt tggtcgacca gtccggacct
     1381 cctcacgagc ccaagttcgt ttaccaagca aaagttgggg gtcgctggtt cccagccgtc
     1441 tgcgcacaca gcaagaagca aggcaagcag gaagcagcag atgcggctct ccgtgtcttg
     1501 attggggaga acgagaaggc agaacgcatg ggtttcacag aggtaacccc agtgacaggg
     1561 gccagtctca gaagaactat gctcctcctc tcaaggtccc cagaagcaca gccaaagaca
     1621 ctccctctca ctggcagcac cttccatgac cagatagcca tgctgagcca ccggtgcttc
     1681 aacactctga ctaacagctt ccagccctcc ttgctcggcc gcaagattct ggccgccatc
     1741 attatgaaaa aagactctga ggacatgggt gtcgtcgtca gcttgggaac agggaatcgc
     1801 tgtgtgaaag gagattctct cagcctaaaa ggagaaactg tcaatgactg ccatgcagaa
     1861 ataatctccc ggagaggctt catcaggttt ctctacagtg agttaatgaa atacaactcc
     1921 cagactgcga aggatagtat atttgaacct gctaagggag gagaaaagct ccaaataaaa
     1981 aagactgtgt cattccatct gtatatcagc actgctccgt gtggagatgg cgccctcttt
     2041 gacaagtcct gcagcgaccg tgctatggaa agcacagaat cccgccacta ccctgtcttc
     2101 gagaatccca aacaaggaaa gctccgcacc aaggtggaga acggagaagg cacaatccct
     2161 gtggaatcca gtgacattgt gcctacgtgg gatggcattc ggctcgggga gagactccgt
     2221 accatgtcct gtagtgacaa aatcctacgc tggaacgtgc tgggcctgca aggggcactg
     2281 ttgacccact tcctgcagcc catttatctc aaatctgtca cattgggtta ccttttcagc
     2341 caagggcatc tgacccgtgc tatttgctgt cgtgtgacaa gagatgggag tgcatttgag
     2401 gatggactac gacatccctt tattgtcaac caccccaagg ttggcagagt cagcatatat
     2461 gattccaaaa ggcaatccgg gaagactaag gagacaagcg tcaactggtg tctggctgat
     2521 ggctatgacc tggagatcct ggacggtacc agaggcactg tggatgggcc acggaatgaa
     2581 ttgtcccggg tctccaaaaa gaacattttt cttctattta agaagctctg ctccttccgt
     2641 taccgcaggg atctactgag actctcctat ggtgaggcca agaaagctgc ccgtgactac
     2701 gagacggcca agaactactt caaaaaaggc ctgaaggata tgggctatgg gaactggatt
     2761 agcaaacccc aggaggaaaa gaacttttat ctctgcccag tatagtaatg aactagtccg
     2821 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattcaagg
     2881 tcgcgacaag atagcga
//
