LOCUS       sequence-283653-        1508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1745
FEATURES             Location/Qualifiers
     source          1..1508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcaact gcggcgcgga cacttgacat tcagggacgt ggccatagaa
       61 ttctctcagg aggagtggaa atgcctggac cctgtgcaga aggctttata cagggatgtg
      121 atgttggaga actacaggaa cctggtctcc ctgggaatct gtcttcctga cctgagtatt
      181 atctccatga tgaagcaaag gacagagccc tggactgtgg agaatgaaat gaaagtagca
      241 aaaaatccag ataggtggga aggtatcaaa gatatcaaca cagggaggag ctgtgcagtg
      301 agaagcaaag caggaaacaa gcctattaca aatcaacttg gattaacctt tcagttacct
      361 ctgccagaac tggagatatt tcaaggtgaa gggaagattt atgaatgtaa tcaagttcaa
      421 aagttcatca gccacagttc ttcagtttcg ccacttcaaa gaatttactc tggggtcaaa
      481 acccacatat ttaataaaca taggaatgat tttgttgatt ttccattgct gtcacaagaa
      541 cagaaagcac acattaggag aaaaccttac gaatgtaatg agcagggcaa agtcttcaga
      601 gtgtcttcaa gccttcctaa tcatcaagta atccacactg cagataaacc taacagatgt
      661 catgaatgtg gtaaaaccgt cagggacaag tcaggcctcg cagaacattg gagaattcgt
      721 acaggagaga aaccttacaa atgtaaagag tgtggcaagc tcttcaatcg aattgcatac
      781 cttgcacgac acgagaaagt gcatactgga gagagtcctt acaaatgtaa tgagtgtggc
      841 aaggtcttca gtcgaattac ataccttgta cgacatcaga aaattcatac tagagagaaa
      901 cctcataaat gtaacaaatg tggcaaggtt tatagtagca gttcatacct agcacaacat
      961 tggagaattc atacaggaga gaaactttac aaatgtaata aatgtggcaa agaatttagt
     1021 gggcattcaa gcctcaccac ccatctgtta atccacactg gagagaaacc ttacaaatgt
     1081 aaagaatgtg acaaagcttt taggcacaag ttctccctga cagttcatca gagaaatcat
     1141 aatggagaga aaccttataa atgtcatgaa tgtggcaaag tcttcactca agtttcacat
     1201 cttgcacgac atcagaaaat tcacactgga gagaaacctt acaaatgtaa tgagtgtggc
     1261 aaagtcttca ctcagaattc acaccttgca aatcatcaga gaatccacac tggagagaaa
     1321 ccttacaaat gccatgtgtg tggtaaggtc tttaggcaca gttcatggtt tgtacagcat
     1381 cagagaagtg ttcatgagag agtccttaca aactagtaat gaactagtcc gtaacttgaa
     1441 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaatgtcg gaacgtgtag
     1501 ggtcaaat
//
