LOCUS       sequence-28366-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-UCHL5
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..25
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     41..57
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    173..197
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
       61 ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg atgacaagct
      121 cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc aaacaagttt
      181 gtacaaaaaa gttggcatga cgggcaatgc cggggagtgg tgcctcatgg aaagcgaccc
      241 cggggtcttc accgagctca ttaaaggatt cggttgccga ggagcccaag tagaagaaat
      301 atggagttta nagcctgaga attttgaaaa attaaagcca gttcatgggt taatttttct
      361 tttcaagtgg cagccaggag aagaaccagc aggctctgtg gttcaggact cccgacttga
      421 cacgatattt tttgctaagc aggtaattaa taatgcttgt gctactcaag ccntagtgag
      481 tgtgttactg aactgtaccc accaggatgt ccatttaggc gagacattat cagagtttaa
      541 agaattttca caaagttttg atgcagctat gaaaggcttg gcactgagca attcagatgt
      601 gattcgacaa gtacacaaca gtttcnccag acagcaaatg tttgaatttg anac
//
