LOCUS       sequence-283668-        3335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1788
FEATURES             Location/Qualifiers
     source          1..3335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagcg tggcggggac gggggcgaga gtgaacgatt caacccgggg
       61 gagctcagga tggcccaaca gcaggccttg aggttccgag gtccggctcc cccaccaaat
      121 gcagtgatgc gaggcccacc acctctgatg cgacctcctc caccttttgg tatgatgcga
      181 ggccctcctc caccaccacg gccgcccttt ggacgtcctc cttttgatcc taatatgccg
      241 ccaatgcctc ctccaggagg gatacctcca cctatgggcc ctccacacct ccagagacca
      301 cctttcatgc ctcctcccat gagttccatg cctcctcctc cgggtatgat gtttccacca
      361 ggaatgcctc ctgtgactgc tcctggtact ccagcactac ctcctacgga ggagatatgg
      421 gttgaaaata aaactccaga tgggaaggtt tattattata atgctcggac acgtgaatct
      481 gcatggacca agccagatgg agttaaggtt attcagcaat cagaactgac acctatgctt
      541 gcagcccagg cacaggttca ggctcaggcc caggcgcagg ctcaggccca ggcgcaggct
      601 caggcccagg cacaagctca ggcccaggct caggctcagg cccaggccca ggcccaggcc
      661 caggcccagg cccaagccca agcccaggcc caggctcagg ctcaggcaca agctcaggcc
      721 caggcccagg ctcaggtcca ggcccaggtc caggcacaag tgcaagcaca agcagttgga
      781 gcttccaccc ctacgaccag tagcccagca cctgcagtat ccacttcaac atcatcatcc
      841 accccttcct ctaccacttc taccacaaca actgctactt cagttgcgca gacagtatca
      901 acacccacaa cacaagatca gaccccaagt tctgctgttt cagttgccac gcctacagtt
      961 agtgtttcaa ctcctgctcc tacagccaca cctgtgcaaa ccgttcccca gccgcaccct
     1021 cagacgttac ctcctgctgt tcctcattca gtacctcagc caacaacagc aatacctgct
     1081 tttccaccag taatggtacc tccgtttcgt gttccccttc ctggcatgcc aattccactt
     1141 ccaggtgtat tgccaggaat ggcccctcct atcgtaccca tgatacatcc ccaggttgct
     1201 attgcagctt cacctgctac cttagctgga gcaacagcag tttctgaatg gactgaatat
     1261 aaaacagcag atgggaagac atattattat aataatagaa cattagaatc aacctgggaa
     1321 aaaccccaag aactaaagga aaaagaaaag ttagaagaga agattaaaga gccaattaaa
     1381 gaaccctctg aagagcctct gccaatggag acggaggagg aggatcctaa agaagagcct
     1441 ataaaggaga taaaggagga gcccaaagaa gaggagatga ctgaagaaga aaaggctgcc
     1501 cagaaggcaa agccagttgc tactgctcct attcctggta ctccatggtg tgtcgtttgg
     1561 actggtgatg agcgggtctt cttttataat cccaccactc gtctttctat gtgggaccga
     1621 cctgatgatc tgattggcag ggcagatgtt gacaaaatta ttcaggagcc ccctcataaa
     1681 aaaggaatgg aggaattgaa gaaactaagg cacccaactc cgacaatgct gtcgatccaa
     1741 aagtggcaat tctctatgag tgcaattaaa gaggaacaag aattaatgga agaaattaat
     1801 gaagatgagc ctgttaaagc aaaaaaacgg aagagagacg ataataaaga cattgactca
     1861 gagaaagaag ctgccatgga agctgaaatt aaagctgccc gagaaagggc cattgtccct
     1921 ctggaggctc gaatgaagca gttcaaggac atgctgctag agagaggggt gtctgctttt
     1981 tcaacgtggg agaaggagtt gcacaagata gtttttgatc cccggtactt acttctcaat
     2041 cctaaagaga gaaaacaggt gtttgatcag tatgtaaaga ccagggcaga ggaagaacgc
     2101 agggaaaaga aaaataaaat aatgcaagcc aaggaagatt tcaaaaaaat gatggaagaa
     2161 gcaaaattta atccaagagc aacttttagt gaatttgcag ccaagcatgc taaagattca
     2221 agattcaaag caattgaaaa gatgaaagac cgagaagcct tgtttaatga gtttgtggcc
     2281 gctgctagga agaaagagaa agaagattcg aagaccagag gtgagaagat taaatcggat
     2341 ttctttgaac tattatctaa tcatcacttg gacagtcagt ctcgatggag caaagtaaaa
     2401 gacaaagtag aaagtgatcc acgttacaaa gcagtagata gttcatcaat gagagaagac
     2461 cttttcaaac agtacattga aaaaatagcc aagaatttag actcagaaaa agaaaaggag
     2521 cttgaaaggc aagcccgcat tgaggcaagc cttcgagaac gagaaaggga ggttcaaaag
     2581 gcccgttcag aacaaacaaa agaaatagat cgagagagag agcagcacaa acgagaagaa
     2641 gctatccaga atttcaaagc tcttctgtct gacatggtac gttcttcaga tgtgtcatgg
     2701 tctgatactc gtaggaccct ccgaaaagat caccgctggg aatctggatc cttattggaa
     2761 agagaggaga aagagaagct ttttaatgaa cacattgaag cacttaccaa aaaaaagaga
     2821 gagcacttta ggcaacttct ggatgaaact tctgcaatta ccttaacatc cacgtggaaa
     2881 gaagtaaaaa aaatcattaa ggaagatcct cgatgtatta agttctcctc cagtgacagg
     2941 aaaaaacaaa gagaatttga agaatatatc agagacaaat atatcacagc caaagctgac
     3001 ttcaggacgc ttttgaaaga gaccaaattt ataacatata gatccaaaaa attaatccaa
     3061 gaatcagatc agcacctgaa agatgtagaa aaaattttac agaatgacaa acggtatcta
     3121 gtactggact gtgtgccaga ggagaggcgt aaactgattg tggcatatgt tgatgacctg
     3181 gatcgccggg gtccaccccc acctcccaca gcatcggagc ccacgagacg atcaacaaaa
     3241 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3301 ggaaaggacg aaaacttact tcgggaatca ggtgt
//
