LOCUS       sequence-283671-        2624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1805
FEATURES             Location/Qualifiers
     source          1..2624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctctcac ccagggacag gtgacattca gggatgtggc tatagaattc
       61 tctcaggagg agtggacatg cctggacccc gctcagagga ctttgtacag ggacgtgatg
      121 ttggagaatt ataggaacct ggcctccctg gcaggaatct cttgttttga cctcagtatt
      181 atctctatgt tggagcaagg gaaggagcct ttcactttgg agagccaagt acaaatagca
      241 ggaaacccag atggatggga atggatcaaa gctgtgatca cagctctctc ttccgaattt
      301 gtaatgaaag atttactaca caaagggaag agtaatacag gagaagtatt ccaaacagtg
      361 atgttggaaa gacaggaaag ccaagacatt gaaggatgtt ccttcaggga agtccagaaa
      421 aatacacatg gccttgagta tcaatgcaga gatgctgaag gaaattacaa aggagtgctt
      481 ttgacccaag aaggcaatct cactcatgga agagatgaac atgataaaag agatgcaaga
      541 aacaagctta ttaaaaatca gcttggatta agccttcagt cacatctgcc tgaactgcag
      601 ctttttcaat atgaagggaa aatttatgaa tgtaatcagg ttgagaagtc tttcaacaat
      661 aattcctcag tttcaccacc tcaacaaatg ccttataatg tcaaaaccca catttctaag
      721 aaatatctca aagattttat ctcttcttta ttactcacac aggggcaaaa agcaaataat
      781 tggggaagcc cttacaaatc taatggatgt ggcatggtct ttcctcaaaa ttcacacctt
      841 gcaagtcatc agagaagtca tactaaagag aaaccttaca aatgttatga gtgtggcaaa
      901 gcctttagaa cacgttcaaa cctaactacc catcaggtga tccatactgg cgaaaaacgt
      961 tacaaatgta atgagtgtgg taaggtcttt agtcgaaatt cacaactctc acaacatcag
     1021 aaaattcaca ctggagagaa accttataaa tgtaacgaat gtggcaaggt cttcactcag
     1081 aattcacacc ttgtaagaca tcgaggaatt catactggag agaaacctta caagtgtaat
     1141 gagtgtggga aagcctttag agctcgttca agcttagcta tccatcaggc aacccacagt
     1201 ggagaaaaac cttacaaatg taatgaatgt ggcaaggtct tcactcaaaa ttcacacctt
     1261 acaaatcact ggagaattca cactggagag aaaccttaca agtgtaatga gtgcggcaaa
     1321 gcctttggtg ttcgttcaag cctagctatt catctggtaa ttcacaccgg agaaaagcct
     1381 tacaaatgtc atgaatgcgg caaggtcttt aggcgtaatt cacaccttgc aaggcatcag
     1441 ctaattcata ctggagagaa accttataag tgtaatgagt gtggcaaagc ctttagagca
     1501 cattcaaacc taactaccca tcaggtcatc catactggag aaaagcctta caaatgtaat
     1561 gaatgtggca aggtcttcac tcaaaattca caccttgcaa atcatcaaag aattcatact
     1621 ggagtgaagc cttatatgtg taatgagtgc ggcaaagcct tcagtgtgta ttcaagccta
     1681 actacccatc aggtcatcca tactggagaa aaaccttaca aatgtaatga gtgtggcaag
     1741 gtcttcactc agaattcaca ccttgcaaga catcggggaa ttcatactgg agagaaacct
     1801 tataaatgta atgaatgtgg caaggtcttt aggcataatt catacctttc aaggcatcag
     1861 cgaattcata ctggagagaa accttacaag tataatgagt atggcaaagc ctttagtgaa
     1921 cattcaaacc taactaccca tcaggtcatc catactggtg aaaaacctta caaatgtaat
     1981 gagtgtggca aggtcttcac tcagaattca caccttgcaa gacatcggag agttcatact
     2041 ggaggtaaac cttaccagtg taatgaatgt ggcaaagcct ttagtcaaac atcaaagctt
     2101 gcaaggcatc agagagttca tactggagag aaaccatatg agtgtaatca gtgtgggaaa
     2161 gcctttagtg tccgttcaag cctaactacc catcaggcaa tccatactgg aaaaaaacct
     2221 tacaaatgca atgagtgtgg gaaggtcttc actcagaatt cacaccttgc aagacatcgg
     2281 ggaattcata ctggagagaa accttacaag tgtaatgagt gtggcaaagc ctttagtcaa
     2341 acttcaaaac ttgcaaggca tcagagaatt cataccggag agaaaccata tgagtgtggg
     2401 aaacccttta gtatctgttc aagcctaact acccatcaga caatccatac tggtgggaaa
     2461 ccttacaaat gtaacgtgtg gaaagttcta aagtcagagt tcaaaccttg caagccatca
     2521 cagaattcat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2581 atatcttgtg gaaaggacga ttggcatcct agcggctgta actg
//
