LOCUS       sequence-283674-        1925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1808
FEATURES             Location/Qualifiers
     source          1..1925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgagaa gcgaaggggc tcgccgtgca gcatgctaag cctcaaggcg
       61 cacgctttct cggtggaggc gctgatcggc gccgagaagc agcaacagct tcagaagaag
      121 cggcgaaaac tgggcgccga agaggcggcg ggggccgtgg acgacggagg ctgcagccgc
      181 ggcggcggcg cgggcgaaaa gggttcttct gagggagacg aaggcgctgc gctcccgccg
      241 ccggctgggg cgacgtctgg gccggctcgg agtggcgcag acctggagcg cggagccgcg
      301 ggcggctgtg aggacggctt ccagcaggga gcttcccctc tggcgtcacc gggaggctcc
      361 cccaaggggt ctccggcgcg ctccctggcc cggcccggga cccctctgcc ctcgccgcag
      421 gccccgcggg tggatctgca gggagccgag ctctggaagc gctttcatga gataggcact
      481 gagatgatca tcaccaaggc cggcaggcgc atgtttccag caatgagagt gaagatctct
      541 ggattagatc ctcaccagca atattacatt gccatggata ttgtaccagt ggacaacaaa
      601 agatacaggt atgtttacca cagttcgaaa tggatggtgg caggtaatgc tgactcgcct
      661 gtgccacccc gtgtgtacat tcatccagac tcgcctgcct cgggggagac ttggatgaga
      721 caagttatca gcttcgacaa gctgaagctc accaacaatg aactggatga ccaaggccat
      781 attattcttc attctatgca caaataccaa ccgcgagtgc acgtcatccg taaagactgt
      841 ggagacgatc tttctcccat caagcctgtt ccatccgggg agggagtaaa ggcattctcc
      901 tttccagaaa ctgtcttcac aaccgtcact gcctatcaga atcagcagat tactcgcctg
      961 aagatagata ggaatccatt tgctaaaggc ttccgagact ccgggcgcaa cagaatgggt
     1021 ttggaagcct tggtggaatc atatgcattc tggcgaccat cactacggac tctgaccttt
     1081 gaagatatcc ctggaattcc caagcaaggc aatgcaagtt cctccacctt gctccaaggt
     1141 actgggaatg gcgttcctgc cactcaccct caccttttgt ctggctcctc ttgctcctct
     1201 cctgccttcc atctggggcc caacaccagc cagctgtgta gtctggcccc tgctgactat
     1261 tctgcctgtg cccgctcagg cctcaccctc aaccgataca gcacatcttt ggcagagacc
     1321 tacaacaggc tcaccaacca ggctggtgag acctttgccc cgcccaggac tccctcctat
     1381 gtgggcgtga gcagcagcac ctccgtgaac atgtccatgg gtggcactga tggggacacc
     1441 ttcagctgcc cacagaccag cttatccatg cagatttcgg gaatgtcccc ccagctccag
     1501 tatatcatgc catcaccctc cagcaatgcc ttcgccacta accagaccca tcagggttcc
     1561 tataatactt ttagattaca cagcccctgt gcactatatg gatataactt ctccacatcc
     1621 cccaaactgg ctgccagtcc tgagaaaatt gtttcttccc aaggaagttt cttggggtcc
     1681 tcaccgagtg ggaccatgac ggatcggcag atgttgcccc ctgtggaagg agtgcacctg
     1741 cttagcagtg ggggtcagca gagtttcttt gactctagga ccctaggaag cttaactctg
     1801 tcatcatctc aagtatctgc acatatggtc tagtaatgaa ctagtccgta acttgaaagt
     1861 atttcgattt cttggcttta tatatcttgt ggaaaggacg atttgttata tattaacaac
     1921 gatcc
//
