LOCUS       sequence-28368-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP12
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatgaaaa tcctaatgac
      121 agtctccaaa ttcgcctcca tctgtaccat gggcgccaat gcttcggcat tagagaaaga
      181 gattggtcca gaacagtttc cggtcaatga gcactatttt ggattagtca attttgggaa
      241 tacctgctac tgcaattcag ttcttcaagc actttatttt tgtcgtccat ttcgggaaaa
      301 agttcttgcg tataagagtc aacctaggaa aaaggagagc cttcttacat gcttagcaga
      361 tctcttccat agcatagcca ctcagaagaa aaaggttgga gtaatacccc ctaagaagtt
      421 catcacaaga ttacggaaag aaaatgagct ttttgacaac tacatgcaac aagatgccca
      481 tgaattctta aattacctac taaatacaat tgctgatatt ttacaagaag agagaaagca
      541 ggaaaaacaa aatggtcgtt tacctaatgg taatattgat aatgaaaata ataacagcac
      601 accagaccca acgtgggttg atgagatttt tcagggaaca ttaactaatg aaaccagatg
      661 tcttacttgt gaaactataa gcagcaaaga tgaagatttt ttagaccttt ctgttgacgt
      721 ggaacaaaat acatcaatta c
//
