LOCUS       sequence-283680-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1823
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgtggc catagagttc tctttggagg agtggcagtg cctggacact
       61 gcacagcgga atgtatatag gcatgtgatg ttagagaact acagaaacct ggttttcttg
      121 gctctgtgtt ctcgttttgc ccaagacctt tggctagagc agaacataaa agattctttc
      181 caaaaagtga cactgagcag atatggaaaa tatggacata agaatttaca gttaagaaaa
      241 ggctgtaaaa gtgtggatga gtgtaaggga caccaaggag gttttaatgg acttaaccaa
      301 tgtttgaaaa ttaccacaag caaaatattt caatgtaata aatatgtaaa agtcatgcat
      361 aaattttcaa attcaaatag acacaagata agacatactg aaaataaaca tttcagatgt
      421 aaagaatgtg acaaatcact ttgcatgctt tcacgcctaa ctcaacataa aaaaattcat
      481 actagagaga atttctacaa atgtgaagag tgtggaaaaa cctttaactg gtccacaaac
      541 ctttctaaac ctaagaaaat tcatactgga gaaaaaccct acaaatgtga agtatgtgga
      601 aaagcctttc accaatcctc aatccttact aaacataaga taattcgtac tggagaaaaa
      661 ccctataaat gtgcacactg tggcaaagcc tttaaacagt cctcacacct tactagacat
      721 aagataattc atactgaaga gaaaccctac aaatgtgaac aatgtggcaa ggtctttaag
      781 cagtccccaa cccttactaa acatcagata atttatactg gagaggaacc atacaaatgt
      841 gaggaatgtg gcaaagcttt taacctatct tagtaatgaa ctagtccgta acttgaaagt
      901 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacgtgcttt catagttaca
      961 ctttg
//
