LOCUS       sequence-28369-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP13
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..90
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     106..122
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    238..262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccntgggtc agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
       61 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
      121 actccttctc taggcgccgg aattagatct gccaccatgg actacaagga tgacgatgac
      181 aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg ttatcaaaca
      241 agtttgtaca aaaaagttgg cgcgcccacc atgcagcgcc ggggcgccct gttcggcatg
      301 ccgggcggca gcggaggcag gaagatggct gcaggagaca tcggcgagct gctagtgccc
      361 cacatgccca cgatccgcgt gcccaggtcc ggcgacaggg tctacaagaa cgagtgcgcc
      421 ttctcctacg actctcccaa ttctgaaggt ggactctatg tatgcatgaa tacatttttg
      481 gcctttggaa gggaacatgt tgaaagacat tttcgaaaaa ctggacagag tgtatacatg
      541 cacctgaaaa gacatgtgcg agagaaggta agaggggcgt ctggtggagc gttaccaaaa
      601 aggaggaatt ccaagatttt tttagatcta gatactgatg acgatttaaa t
//
