LOCUS       sequence-283695-        1433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1859
FEATURES             Location/Qualifiers
     source          1..1433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             292..321
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             295..324
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             298..327
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             304..333
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tgccctgcgt ccaagcccaa tatagccctt cccctccagg ttccagttat
       61 gcggcgcaga catacagctc ggaatacacc acggagatca tgaaccccga ctacaccaag
      121 ctgaccatgg accttggcag cactgagatc acggctacag ccaccacgtc cctgcccagc
      181 atcagtacct tcgtggaggg ctactcgagc aactacgaac tcaagccttc ctgcgtgtac
      241 caaatgcagc ggcccttgat caaagtggag gaggggcggg cgcccagcta ccatcaccat
      301 caccaccacc accaccacca ccaccaccat caccagcagc agcatcagca gccatccatt
      361 cctccagcct ccagcccgga ggacgaggtg ctgcccagca cctccatgta cttcaagcag
      421 tccccaccgt ccacccccac cacgccggcc ttccccccgc aggcgggggc gttatgggac
      481 gaggcactgc cctcggcgcc cggctgcatc gcacccggcc cgctgctgga cccgccgatg
      541 aaggcggtcc ccacggtggc cggcgcgcgc ttcccgctct tccacttcaa gccctcgccg
      601 ccgcatcccc ccgcgcccag cccggccggc ggccaccacc tcggctacga cccgacggcc
      661 gctgccgcgc tcagcctgcc gctgggagcc gcagccgccg cgggcagcca ggccgccgcg
      721 cttgagagcc acccgtacgg gctgccgctg gccaagaggg cggccccgct ggccttcccg
      781 cctctcggcc tcacgccctc ccctaccgcg tccagcctgc tgggcgagag tcccagcctg
      841 ccgtcgccgc ccagcaggag ctcgtcgtct ggcgagggca cgtgtgccgt gtgcggggac
      901 aacgccgcct gccagcacta cggcgtgcga acctgcgagg gctgcaaggg ctttttcaag
      961 agaacagtgc agaaaaatgc aaaatatgtt tgcctggcaa ataaaaactg cccagtagac
     1021 aagagacgtc gaaaccgatg tcagtactgt cgatttcaga agtgtctcag tgttggaatg
     1081 gtaaaagaag ttgtccgtac agatagtctg aaagggagga gaggtcgtct gccttccaaa
     1141 ccaaagagcc cattacaaca ggaaccttct cagccctctc caccttctcc tccaatctgc
     1201 atgatgaatg cccttgtccg agctttaaca gactcaacac ccagagatct tgattattcc
     1261 agagtaagtt ttatgatttc ctgctttcaa atgaatgatc agggtctcta tttatggcta
     1321 ctagtaataa gagttgatta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1381 tggctttata tatcttgtgg aaaggacgag caattccgga tgctttgtcg tgg
//
