LOCUS       sequence-283710-        1799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1948
FEATURES             Location/Qualifiers
     source          1..1799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatggggc ttcttcaggc ggtggcggca gcagcgaagg tggcggcggc
       61 agcagcggca gcggctatgg tgtggtcgct cgattctccc agtgcctggc tgagtttcgg
      121 acgtggttaa gaaccaactg gttgaggttc aatgcagaca agacggatgt gatgctgcca
      181 tctgttgaat cttcaagtcc aggaggttca gcaacatcag atgaccatga atttgatcca
      241 tcagctgaca tgctggttca tgattttgat gatgaacgaa cattagaaga ggaagaaatg
      301 atggaaggag aaacaaactt cagctctgaa atagaagatc ttgcaaggga aggcgacatg
      361 ccaattcatg aacttctcag cctttatggt tatggtagta ctgttcgact acctgaagaa
      421 gatgaggaag aggaagaaga ggaagaagaa ggtgaagatg atgaagatgc tgataatgat
      481 gacaacagtg gctgtagtgg ggaaaataaa gaggagaata taaaggattc atcaggtcag
      541 gaggatgaaa ctcagtcttc caatgatgat ccatcacaat ctgttgcttc tcaagatgcc
      601 caggaaataa tccgcccacg tcgatgtaaa tattttgata caaatagtga agtagaagaa
      661 gaatctgaag aagatgaaga ttatattcca tcagaagact ggaaaaagga gattatggtg
      721 ggctccatgt ttcaagcaga aattccagtt ggcatttgta gatacaaaga aaatgaaaaa
      781 gtatatgaaa atgatgatca gctcctgtgg gaccctgagt acttaccaga agataaagtg
      841 attatatttc ttaaagatgc atctagaaga acaggtgatg agaagggtgt agaagcaatt
      901 cctgaaggat ctcacataaa agacaatgaa caggctttat atgaattggt taaatgcaat
      961 tttgatacag aagaagcatt gagaagatta agatttaatg taaaagcagc tagagaggaa
     1021 ttatctgttt ggacagagga agagtgtaga aattttgaac aagggctgaa ggcctatgga
     1081 aaggattttc atttgattca ggctaataaa gtccgaacaa ggtcagttgg tgaatgtgta
     1141 gcattctatt acatgtggaa aaaatctgaa cgttatgatt tctttgctca gcaaacacga
     1201 tttggaaaga agaaatataa tcttcatcct ggtgtaacgg attacatgga tcgtcttcta
     1261 gacgaaagtg aaagtgctgc atctagtcga gcaccatccc ctcccccaac tgcatcaaac
     1321 agtagtaaca gccagtctga gaaagaagat ggcactgtaa gcactgctaa tcaaaatgga
     1381 gtgtcatcta atggaccagg tgaaatatta aacaaagagg aagtaaaagt tgaagggtta
     1441 cacattaatg gaccaacagg tggaaataag aaaccacttc atgcagatat ggatactaat
     1501 ggttatgaaa cagataacct taccactgac ccaaaacttg cccatatgac tgcaagaaat
     1561 gaaaatgatt ttgatgaaaa aagtgagaga cctgccaaaa ggcgaagggt aaacagcaat
     1621 ggaaaagaaa gtccaggttc ttctgaattt ttccaagaag cagtctcaca tgggaaattt
     1681 gaagaacttg aaaacacaga tgactagtaa tgaactagtc cgtaacttga aagtatttcg
     1741 atttcttggc tttatatatc ttgtggaaag gacgacagga gttgcggtag agcccaatt
//
