LOCUS       sequence-283716-        2087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1986
FEATURES             Location/Qualifiers
     source          1..2087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcaggggcaa ttgtcattca gcgatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gggcagaaag ctttatacag ggacgtgatg
      121 ttagagaact acaggaacct ggtctcccta ggaatctgtc ttcctgacct gagtgttacc
      181 tccatgttgg agcaaaagag agatccctgg actctgcaga gtgaagtgaa aataataaac
      241 aatccagatg gcagggagtg catcaaaggt gtgaacacag agaagagttc taaattggga
      301 agcagtgcag gaaacaagtc acttaaaaat caacatggat taactcttca gttacatctg
      361 actgaatggc agccatttca agctgtaagg aatatttatg gatgtaagca tgttgagaaa
      421 tctatcagtg acaattcttc agtttcacca gttcaaataa gttttttcag tgtcaaaacc
      481 catattttta ataactacag aaatgatttt cttttttcta cattactccc acaagaacag
      541 aaagtacaca ttagggaaaa gccttatgga tgtaatgagc atgggaaagt cttcagagtg
      601 tcttcaagcc ttactaaccg tcaagtaatc cacattgcag ataaaactta caaatgtagt
      661 gactgtggcg agatctttag tagcaattca aactttgcac aacatcaacg aatccatact
      721 ggagagaagc cttacaaata taatgaatgt ggcaaagtct tcaatcagaa ttcacacctt
      781 gcacaacatc agaaaattca tactggacag aaaccttaca ataacaaaga atgtgggaaa
      841 gtctttagtc accatgccta ccttgcacag cataggaaaa ttcatactgg agagaagcct
      901 tacaaatgta gtgaatgtgg caaagcattt agtgtgtgtt ccagtcttac tgctcatctt
      961 gtaatccata ctggagagaa accttatgat tgtaaggaat gtggcaaggt cttcaggcat
     1021 aagtcttccc taaccactca tcagacagtt catactggag agagaccata caaatgtaat
     1081 gaatgtggca aggggtttag tcgaattgca ttccttgcaa ggcatcggaa agttcatact
     1141 ggagagaaac cttacaaatg taatgagtgt ggcaaggtct ttattggcaa ttcacgcctt
     1201 gcacgacata ggaaaattca tactgggggg aggcgttaca aatgtaatga atgtggcaaa
     1261 gcatttagaa cgtgttcaga tctcactgcc catcttctaa tccatactgg agagaaacct
     1321 tacgaatgta tagactgtgg caaggtcttc aggcacaagt cttccctaac ctatcactgt
     1381 agaattcata ctggagagaa gccttacaaa tgtaacgaat gtggcaaggt cttcagtcag
     1441 aattcaaacc ttcaacgaca taggaaaatt catactggag agaagcttta caaatgtaat
     1501 gaatgtggca aggtcttccg tcagaattca caccttgcac aacataggga tattcatact
     1561 ggagagaagc cttacagttg taatgaatgt ggcaaggtct tccgtcggaa ttcacacctt
     1621 gtgcgacata ggaatgttca tactggagaa aagccttaca gttgtaatga atgtggcaag
     1681 gtcttcagtc ggaattcaca ccttgcgcga cataggaata ttcatactgg agagaagcct
     1741 cacagttgta atgaatgtgg caaggtcttc agtcggaatt cacaccttgc gcgacatagg
     1801 aaaattcata ctggagagaa gctttacaaa tgtaatgaat gtagcaaggt cttcagtcgg
     1861 aattcacgcc ttgcacaaca taggaatatt catactggag tgaagcctta cagttgtaat
     1921 gaatgtggca aggtctttag taaaaattcc atcctagtac aacattgcag tattcatacc
     1981 agagagaagc cttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2041 tatatatctt gtggaaagga cgagttctat tggaacacgg gccaggc
//
