LOCUS       sequence-28372-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP15
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatggcgg aaggcggagc
      121 ggcggatctg gacacccagc ggtctgacat cgcgacgctg ctcaaaacct cgctccggaa
      181 aggggacacc tggtacctag tcgatagtcg ctggttcaaa cagtggaaaa aatatgttgg
      241 ctttgacagt tgggacaaat accagatggg agatcaaaat gtgtatcctg gacccattga
      301 taactctgga cttctcaaag atggtgatgc ccagtcactt aaggaacatc ttattgatga
      361 attggattac atactgttgc caactgaagg ttggaataaa cttgtcagct ggtacacatt
      421 gatggaaggt caagagccaa tagcacgaaa ggtggttgaa cagggtatgt ttgtaaagca
      481 ctgcaaagta gaagtatatc tcacagaatt gaagctatgt gaaaatggaa acatgaataa
      541 tgttgtaact cgaagattta gcaaagctga cacaatagat acaattgaaa aggaaataag
      601 aaaaatcttc agtattccag atgaaaagga gaccagattg tggaacaaat acatgagtaa
      661 cacatttgaa ccactgaata aaccagac
//
