LOCUS       sequence-283725-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2049
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaccg agacagcggc agcgagcagg gtggtgcggc gctgggttcg
       61 ggcggctccc tggggcaccc cggctcgggc tcaggctccg gcgggggcgg tggtggcggc
      121 gggggcggcg gcggcagtgg cggcggcggc ggcggggccc caggggggct gcagcacgag
      181 acgcaggagc tggcctccaa gcgggtggac atccagaaca agcgcttcta cctggacgtg
      241 aagcagaacg ccaagggccg cttcctgaag atcgccgagg tgggcgcggg cggcaacaag
      301 agccgcctta ctctctccat gtcagtggcc gtggagttcc gcgactacct gggcgacttc
      361 atcgagcact acgcgcagct gggccccagc cagccgccgg acctggccca ggcgcaggac
      421 gagccgcgcc gggcgctcaa aagcgagttc ctggtgcgcg agaaccgcaa gtactacatg
      481 gatctcaagg agaaccagcg cggccgcttc ctgcgcatcc gccagacggt caaccggggg
      541 cctggcctgg gctccacgca gggccagacc attgcgctgc ccgcgcaggg gctcatcgag
      601 ttccgtgacg ctctggccaa gctcatcgac gactacggag tggaggagga gccggccgag
      661 ctgcccgagg gcacctcctt gactgtggac aacaagcgct tcttcttcga tgtgggctcc
      721 aacaagtacg gcgtgtttat gcgagtgagc gaggtgaagc ccacctatcg caactccatc
      781 accgtgccct acaaggtgtg ggccaagttc ggacacacct tctgcaagta ctcggaggag
      841 atgaagaaga ttcaagagaa gcagagggag aagcgggctg cctgtgagca gcttcaccag
      901 cagcaacagc agcagcagga ggagaccgcc gctgccaccc tgctactgca gggtgaggaa
      961 gaaggggaag aagattagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1021 ctttatatat cttgtggaaa ggacgaacta aacctagcat aatctgttag
//
