LOCUS       sequence-28374-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP18
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..22
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             35..61
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctacaaggat gacgatgaca agctcgatgg aggataccca tacgatgttc cagattacgc
       61 tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc atgagcaagg cgtttgggct
      121 cctgaggcaa atctgtcagt ccatcctggc tgagtcctcg cagtccccgg cagatcttga
      181 agaaaagaag gaagaagaca gcaacatgaa gagagagcag cccagagagc gtcccagggc
      241 ctgggactac cctcatggcc tggttggttt acacaacatt ggacagacct gctgccttaa
      301 ctccttgatt caggtgttcg taatgaatgt ggacttcacc aggatattga agaggatcac
      361 ggtgcccagg ggagctgacg agcagaggag aagcgtccct ttccagatgc ttctgctgct
      421 ggagaagatg caggacagcc ggcagaaagc agtgcggccc ctggagctgg cctactgcct
      481 gcagaagtgc aacgtgccct tgtttgtcca acatgatgct gcccaactgt acctcaaact
      541 ctggaacctg attaaggacc agatcactga tgtgcacttg gtggagagac tgcaggccct
      601 gtatacgatc cgggtgaagg actccttgat ttgcgttgac tgtgccatgg agagtagcag
      661 aaacagcagc atgctcaccc tcccactttc tctttttgat gtggactcaa agcccctgaa
      721 gacact
//
