LOCUS       sequence-283743-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2172
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc aggttcctgt ggctgcagac
       61 ttgcttacag accatgagga gggctatgtg acctttgagg atgtggctgt ctacttctcc
      121 caggaggaat ggagattgct tgatgacgct cagaggctcc tctaccgcaa tgtgatgctg
      181 gagaacttta cacttctggc ctctctggga cttgcatctt ccaagaccca tgaaataacc
      241 cagctggagt catgggagga gcccttcatg cctgcttggg aagttgtgac ttcagccata
      301 ccgagaggtt gttggcatgg agccgaggct gaggaggctc ctgagcagat tgcttctgta
      361 ggactgctca gttcaaacat tcagcaacac cagaagcagc actgtggaga gaaaccctta
      421 aaaagacaag agggcagggt cccagttttg aggagttgca aagttcacct atcagagaag
      481 tccttgcaaa gcagggaggt tgggaaggcc ctcctgatca gctcaggtgt tctcaagcac
      541 caggtgactc acacaggaga gaagtcacat aggagctcca aaagtaggga ggcctttcat
      601 gctggaaaaa ggcattacaa atgcagtgaa tgtgggaaag cctttggtca gaaatattta
      661 cttgttcagc accagagact acatgctggg aaaaagacgt atgaatgcag tgaatgtggg
      721 aagttattta gagatatgtc caaccttttt atacaccaaa tagttcacac tggagaaagg
      781 ccttacgggt gtagtaactg tggaaaatcc tttagccgta atgctcacct cattgaacac
      841 cagagagttc acactggaga aaagcctttt acatgcagtg aatgtggaaa agctttcagg
      901 cataattcca cacttgttca gcatcacaaa atccacactg gagtaaggcc ttatgagtgc
      961 agtgaatgtg gaaaattgtt tagtttcaac tccagcctca tgaaacatca gagaattcac
     1021 actggagaaa gaccttataa gtgcagtgaa tgtggaaaat tctatagcca caagtccaac
     1081 cttatcaaac attggcgtgt tcatactgga gaaaggcctt acaagtgcag cgactgtggg
     1141 aaatttttta cccaatgctc aagcctcatg caacatcaaa aagttcacac tggagaaaag
     1201 ccttttaagt gcaatgaatg tgggagattc tttagagaga attccaccct agttagacat
     1261 cagagggttc acactggagc aaagccttat gagtgcaggg aatgtgggaa attttttagc
     1321 caaagctcaa ccctcatgca acatcgaaaa gttcacattg gagaaaagcc ttttaagtgc
     1381 aatgaatgtg ggagattgtt tagagagaat tccagccttg ttaaacatca gagggttcac
     1441 actggagcaa agccttatga gtgcagggaa tgtgggaagt tttttcgcca caactccagt
     1501 ctttttaaac atcgaaggat tcacactgga gaaatgcagt agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aactttacag
     1621 gaccttcaga cctc
//
