LOCUS       sequence-283746-        5822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0065
FEATURES             Location/Qualifiers
     source          1..5822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgattgaaga caaggggcct cgtgttgctg actactttgt tgtagcagga
       61 ttaactgatg tttcaaagcc tctagaagaa gaaattcact tcaatgatgc ttgtcataaa
      121 gtagctaaac caaaagaacc tattacagat gtttcagtta ttatcaaatc tcttggggag
      181 gaagtcccac aggattatat ctgtattgat gttaccccaa ctggattgtc agctgatctt
      241 aataatggaa gtcttgtggg gccacagatt tacctttgct atagaagagg aagagataag
      301 cctccactta cagatctggg ggttttatat gactggaaag aaagattgaa acagggttgt
      361 gaaattattc agagtactcc ctatgggcgc cccgcaaata ttagtgggag tacctcatca
      421 caaagaattt atatcactta ccgaagagcc tctgaaaaca tgactcagaa tacgttggct
      481 gtcactgaca tatgtattat tatacccagt aaaggagaaa gcccaccaca cacgttctgc
      541 aaagtcgaca agaatctcaa taacagtatg tggggatcag cagtatactt gtgttataaa
      601 aaatcggtgg caaagacgaa cactgtatct tacaaagctg gcctaatttg tagatatcct
      661 caagaagatt atgagtcatt ctcactaccg gaatctgttc ctctattttg tttaccaatg
      721 ggtgcaacca ttgaatgttg gccatcaaat agcaaatacc ctctgcctgt attttctact
      781 tttgttttaa ctggagcctc agctgaaaag gtttatggtg ctgctattca gttttatgaa
      841 ccatactctg aggagaatct cacagaaaag cagagacttc ttttgggttt aacatcagca
      901 gatgggaagt ctgatagttc caaaacaatt catactaaca aatgcatctg tcttctttct
      961 cactggcctt tttttgatgc attcaggaag tttctgactt ttctgtatcg ttattccatc
     1021 tctgggcctc atgtccttcc aattgagaag catatttctc attttatgca taaagttcct
     1081 tttccatctc ctcagagacc acggatttta gttcagttat caccacatga taatctgatt
     1141 ctcagtcagc ctgtgtcttc acctcttcca ctaagtggtg gcaagttttc aacactactg
     1201 cagaatttag gccctgaaaa tgctgtgaca ctactggtgt ttgcagtaac agaacataaa
     1261 attcttatcc attccctacg gccatccgtg cttactagtg tgacagaagc attagtgtct
     1321 atgattttcc ctttccactg gccatgcccg tatgttcctc tctgcccact ggctttagca
     1381 gatgtcttga gtgcaccatg tccattcata gtagggattg attcaagata ctttgatctc
     1441 tatgatcctc caccagatgt cagttgtgtt gatgtagata ccaacactat ttctcaaatt
     1501 ggtgacaaga aaaatgtagc ctggaagata cttccaaaga agccatgtaa aaatctgatg
     1561 aacacactga ataatttgca tcagcaattg gcaaaattgc agcagagacc gagagatgat
     1621 ggactcatgg atttagcaat aaatgactat gattttaatt ctggaaagag gttgcacatg
     1681 atagatttgg aaatccaaga ggcatttttg tttttcatgg cctctatttt aaaaggttac
     1741 agatcatact taaggccaat aacacaagcc ccatctgaga cagccacaga tgcagcctct
     1801 ctctttgccc tacaagcttt cttaagaagc cgggaccggt cacatcaaaa attctataac
     1861 atgatgacca aaacacaaat gtttattcgc ttcattgaag aatgttcttt tgttagtgac
     1921 aaagatgcaa gcctggcatt ttttgatgat tgtgtagata aagtggatat ggacaagagc
     1981 ggtgaagtac gacttataga actggatgaa tctttcaaaa gtgaacacac tgtttttgta
     2041 acaccacctg agatccccca cctacccaat ggtgaagaac cccctttaca gtacagctat
     2101 aatggatttc cagttcttag gaacaattta tttgaaagac ctgaaggatt tctacaagca
     2161 aagaagaaca aattgccttc aaaatcaagt agtcctaata gccctttgcc catgttcaga
     2221 agaacaaaac aggaaattaa atcagcccat aaaattgcaa agaggtactc ttccatccct
     2281 cagatgtggt ctaggtgtct actgcgccac tgttatggac tgtggtttat ttgtctccca
     2341 gcttatgtga aagtctgtca ttcaaaagtc agggctctga aaacagcata tgatgtgctt
     2401 aaaaaaatgc agtcaaagaa gatggatcca cctgatgagg tatgctaccg cattcttatg
     2461 caactctgtg gacaatatga tcagccagtg cttgcagttc gagtgctttt tgaaatgcag
     2521 aaagctggta ttgaccccaa tgccattact tatggttatt ataataaggc tgttttggaa
     2581 agtacctggc cttcaagaag tcgtagtggc tattttcttt ggacaaaagt aagaaatgtt
     2641 gttttaggag taacacagtt caaaagagct ttaaagaagc atgcacactt atcacaaaca
     2701 actctctcag atggcagtga cctggatgct gttagtcatg gcagcatgga tagtggtcat
     2761 gggacacaca ctgtggagca ggcacctttt aatacgggtt taatcaaagt atatgctact
     2821 gatgatagat ctagtacagg tggccagtct gatcttggat ataattcatt atctaaagat
     2881 gaagttagaa gaggggatac atctactgaa gacattcaag aagaaaaaga taaaaaaggg
     2941 agtgattgta gttccttgtc agagagtgag agtacaaaag gaagtgctga ttgccttcct
     3001 aagctcagtt atcaaaattc ttccagtatc gttcgcctca ctggtacaag taacaacagt
     3061 gctggtaaaa tatcaggaga aagcatggaa agcacacctg agctgctctt aatatcatct
     3121 cttgaagata caaatgaaac acgaaacatt caaagtagat gcttcaggaa aagacataaa
     3181 agtgacaatg aaactaattt gcagcagcaa gtggtctggg gaaatagaaa ccgtaatctt
     3241 agtggagggg tactgatggg atttatgctc aatagaatta accaggaagc aacccctgga
     3301 gatatagttg aaaaattggg agctgatgca aaaattcttt caaatgttat ctcaaaaagc
     3361 acgagaccaa atactcttga tattgggaag ccacctttaa gatcaaaaag agacagtcta
     3421 gaaaaggaat ctagtgatga tgatacacct tttgatggtt ctaactattt ggcagataaa
     3481 gtggattctc ctgttatttt tgacttagaa gacttagaca gtgaaacaga tgtatcaaaa
     3541 gcaggatgtg ttgctacaca aaatcccaag aggattcaac gtatgaacag cagcttttca
     3601 gtaaaacctt ttgaaaaaac tgatgtcgca acaggatttg atcccctctc tcttttggtt
     3661 gctgagactg aacagcagca aaaagaagaa gaggaggagg atgaagatga tagcaaaagc
     3721 atttcaacac catctgctag gcgtgattta gctgaagaaa ttgtgatgta tatgaataac
     3781 atgagcagtc ctttgacaag tcgtacacca agcattgatt tacaacgggc atgtgatgat
     3841 aaattaaata agaaaagtcc accattggtc aaggcatgca gaagatctag tctgcctcct
     3901 aattctccta agccagtgag acttaccaaa tctaaaagtt acacaaaaag tgaggagaaa
     3961 ccaagggata gactttggtc ttctccagcc ttctcaccta cttgcccatt tagggaagaa
     4021 tctcaggata cattaaccca ctcatcacct tcatttaact tagatacact actagtacct
     4081 aaactagatg ttctaagaaa cagtatgttc actgctggaa aaggagttgc agagaaagca
     4141 agcaaatggt attcaagatt caccatgtac accacatcat ctaaggatca aagttctgat
     4201 cgtaccagtc tttcttcagt gggagcccag gattctgaat ctacctcttt aacagatgaa
     4261 gatgtctgcc atgagttgga aggacctatc tcctctcaag agaccagtgc tacttcaggg
     4321 actaagagaa ttgatctcag ccgaataagc ctggaaagtt ctgcatcctt ggaaggatct
     4381 ctgtcgaagt ttgccttacc tgggaaatca gaagtgacat cttccttcaa cgcgagtaat
     4441 acaaatatct tccagaacta tgcaatggag gttctcatct caagttgctc tcggtgtaga
     4501 acttgtgatt gtcttgtcca tgatgaggaa atcatggctg gctggacagc agatgattca
     4561 aatctcaata ctacatgccc attctgtggc aatatcttct taccctttct gaatatagaa
     4621 ataagagatt taagacgacc tggaagatac tttctaaagt caagcccatc aacagaaaat
     4681 atgcactttc catcctccat ttcaagtcag acgaggcagt cttgcatttc aacatcagcc
     4741 tctggtcttg acacatctgc tctctctgtt caagggaatt ttgatctaaa tagcaaatct
     4801 aaactgcagg aaaatttttg cacccgaagt attcagatcc ctgctaatag atcaaaaaca
     4861 gctatgtcta aatgtccaat atttccaatg gccaggagta ttagtacttc tggccccttg
     4921 gataaggaag atactggaag acagaagctc atttctacag gcagcctgcc agctacttta
     4981 caaggagcta cagattcttt aggtttagaa tggcaccttc caagtcccga tcctgtcact
     5041 gttccgtatc ttagtccttt agtggtatgg aaagaacttg aaagcttatt ggaaaatgaa
     5101 ggtgatcatg caataacagt agcagacttt gtggaccatc atccaattgt tttttggaac
     5161 ctggtatggt atttcagacg tcttgacttg cccagtaact tacctggatt gattctttct
     5221 tctgagcact gtaacaagta ttcaaagatt ccccgccact gtatgtctga agatagcaaa
     5281 tatgttttaa tacaaatgtt atgggacaat atgaagttac atcaggatcc gggacagccc
     5341 ttgtacattc tctggaatgc acacacccaa aaatatccaa tggtccattt attgcaaaaa
     5401 agtgataact catttaacca ggaactgttg aaaagtatgg taaaaagcat taaaatgaat
     5461 gatgtctatg gaccaatgag tcagatttta gagacactga ataagtgtcc acattttaaa
     5521 agacagagga gtttatacag agaaatacta tttctctcac ttgtggcact gggaagagaa
     5581 aacattgata tagatgcttt tgataaagag tacaagatgg catacgatcg tctcacacct
     5641 agtcaagtca agagtacaca caactgtgat agaccaccaa gtacaggggt gatggaatgt
     5701 cgaaaaacct ttggagaacc ttatctttag taatgaacta gtccgtaact tgaaagtatt
     5761 tcgatttctt ggctttatat atcttgtgga aaggacgaca gatgaaagca ctgtatctgt
     5821 ac
//
