LOCUS       sequence-283755-        2018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0420
FEATURES             Location/Qualifiers
     source          1..2018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaaacct ccagacaaat ttctccttgg ttcagggctc aactaaaaaa
       61 ctgaatggga tgggagatga tggcagcccc ccagcgaaaa aaatgataac ggacattcat
      121 gcaaatggaa aaacgataaa caaggtgcca acagttaaga aggaacactt ggatgactat
      181 ggagaagcac cagtggaaac tgatggagag catgttaagc gaacctgtac ttctgttcct
      241 gaaactttgc atttaaatcc cagtttgaaa cacacattgg cacaattcca tttaagtagt
      301 cagagctcgc tgggtggacc agcagcattt tctgctcggc attcccaaga aagcatgtcg
      361 cctactgtat ttctgcctct tccatcacct caggttcttc ctggcccatt gctcatccct
      421 tcagatagct ccacagaact cactcagact gtgttggaag gggaatctat ttcttgtttt
      481 caagttggag gagaaaagag actctgtttg ccccaagtct taaattctgt tctccgagaa
      541 tttacactcc agcaaataaa tacagtgtgt gatgaactgt acatatattg ttcaaggtgt
      601 acttcagacc agcttcatat cttaaaggta ctgggcatac ttccattcaa tgccccatcc
      661 tgtgggctga ttacattaac tgatgcacaa agattatgta atgctttatt gcggccacga
      721 acttttcctc aaaatggtag cgtacttcct gctaaaagct cattggccca gttaaaggaa
      781 actggcagtg cctttgaagt ggagcatgaa tgcctaggca aatgtcaggg tttatttgca
      841 ccccagtttt atgttcagcc tgatgctccg tgtattcaat gtctggagtg ttgtggaatg
      901 tttgcacccc agacgtttgt gatgcattct cacagatcac ctgacaaaag aacttgccac
      961 tggggctttg aatcagctaa atggcattgc tatcttcatg tgaaccaaaa atacttagga
     1021 acacctgaag aaaagaaact gaagataatt ttagaagaaa tgaaggagaa gtttagcatg
     1081 agaagtggaa agagaaatca atccaagaca gatgcaccat caggaatgga attacagtca
     1141 tggtatcctg ttataaagca ggaaggtgac catgtttctc agacacattc atttttacac
     1201 cccagctact acttatacat gtgtgataaa gtggttgccc caaatgtgtc acttacttct
     1261 gctgtatccc agtctaaaga gctcacaaag acagaggcaa atgcatcaat ctcaaataat
     1321 tctacaagta aaaggaaatc tgagtctgcc acttgcaact tagtcagaga cataaacaaa
     1381 gtgggaattg gccttgttgc tgccgcttca tctccgcttc ttgtgaaaga tgtcatttgt
     1441 gaggatgata agggaaaaat catggaagaa gtaatgagaa cttatttaaa acaacaggaa
     1501 aaactaaact tgattttgca aaagaagcaa caacttcaga tggaagtaaa aatgttgagt
     1561 agttcaaaat ctatgaagga actcactgaa gaacagcaga atttacagaa agagcttgaa
     1621 tctttgcaga atgaacatgc tcaaagaatg gaagaatttt atgttgaaca gaaagactta
     1681 gagaaaaaat tggagcagat aatgaagcaa aaatgtacct gtgactcaaa tttagaaaaa
     1741 gacaaagagg ctgaatatgc aggacagttg gcagaactga ggcagagatt ggaccatgct
     1801 gaggccgata ggcaagaact ccaagatgaa ctcagacagg aacgggaagc aagacagaag
     1861 ttagagatga tgataaaaga gctaaagctg caaattctga aatcatcaaa gactgctaaa
     1921 gaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1981 tgtggaaagg acgataacta cgttgatggc ttatccac
//
