LOCUS       sequence-28377-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP22
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatggtgt cccggccaga
      121 gcccgagggc gaggccatgg acgccgagct ggcggtagcg ccgccgggct gctcgcacct
      181 gggcagcttc aaggtggaca actggaagca gaacctgcgg gccatctacc agtgcttcgt
      241 gtggagcggc acggctgagg cccgcaagcg caaggccaag tcctgtatct gccatgtctg
      301 tggcgtccac ctcaacaggc tgcattcctg cctctactgt gtcttcttcg gctgtttcac
      361 aaagaagcat attcacgagc atgcgaaggc gaagcggcac aacctggcca ttgatctgat
      421 gtacggaggc atctactgtt ttctgtgcca ggactacatc tatgacaaag acatggaaat
      481 aatcgccaag gaggagcagc gaaaagcttg gaaaatgcaa ggcgttggag agaagttttc
      541 aacttgggaa ccaaccaaac gggagcttga actgctgaag cacaacccga aaaggagaaa
      601 gatcacctcg aactgcacca taggtctgcg tgggctgatc aaccttggga acacatgctt
      661 catgaactgc atcgtgcagg ccctgaccca cacgccactt ctgcgggact tcttcctgtc
      721 tgacaggcac cgctgtgaga tgcanagccc cagctcctgt c
//
