LOCUS       sequence-283773-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0514
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttaga gaaaaattat ccgactcctc ggaccagcag gacaggacat
       61 ggaggagtga atcagcttgg gggggttttt gtgaatggac ggccactccc ggatgtagtc
      121 cgccagagga tagtggaact tgctcatcaa ggtgtcaggc cctgcgacat ctccaggcag
      181 cttcgggtca gccatggttg tgtcagcaaa attcttggca ggtattatga gacaggaagc
      241 atcaagcctg gggtaattgg aggatccaaa ccaaaggtcg ccacacccaa agtggtggaa
      301 aaaatcgctg aatataaacg ccaaaatccc accatgtttg cctgggagat cagggaccgg
      361 ctgctggcag agcgggtgtg tgacaatgac accgtgccta gcgtcagttc catcaacagg
      421 atcatccgga caaaagtaca gcagccaccc aaccaaccag tcccagcttc cagtcacagc
      481 ataggtattc aggagtctcc ggtgccgaac ggccactcgc ttccgggcag agacttcctc
      541 cggaagcaga tgcggggaga cttgttcaca cagcagcagc tggaggtgct ggaccgcgtg
      601 tttgagaggc agcactactc agacatcttc accaccacag agcccatcaa gcccgagcag
      661 accacagagt attcagccat ggcctcgctg gctggtgggc tggacgacat gaaggccaat
      721 ctggccagcc ccacccctgc tgacatcggg agcagtgtgc caggcccgca gtcctacccc
      781 attgtgacag gccgtgactt ggcgagcacg accctccccg ggtaccctcc acacgtcccc
      841 cccgctggac agggcagcta ctcagcaccg acgctgacag ggatggtgcc tggctccccc
      901 tactattata gcgctgccgc ccgaggagcc gccccacctg cagccgccac tgcctatgac
      961 cgtcactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1021 tcttgtggaa aggacgatgg aacatccgag aaccaccatt t
//
