LOCUS       sequence-283779-        1955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0556
FEATURES             Location/Qualifiers
     source          1..1955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1250..1258
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgatcaagtc ccaggaatca ctgaccctgg aggatgtggc tgtggagttc
       61 acttgggagg agtggcagct cctcggccct gctcagaagg acctgtaccg agacgtgatg
      121 ttggagaact atagcaacct cgtgtcagtg gggtatcaag ccagcaaacc agatgcactc
      181 ttcaagttgg aacaaggaga gccatggaca gtagaaaatg aaatccacag ccaaatctgt
      241 ccagaaatca agaaagttga caatcatcta cagatgcact cacaaaagca aagatgtctg
      301 aagagagtgg aacaatgcca taaacataat gcatttggaa acatcattca tcagaggaaa
      361 agtgattttc ctttaaggca aaatcatgat acatttgact tacatgggaa aatactgaaa
      421 tcaaatttaa gtttagtcaa ccagaacaaa aggtatgaaa tcaagaattc tgtgggggtt
      481 aatggagatg ggaaatcctt ccttcatgcc aagcatgaac aatttcataa tgaaatgaac
      541 ttccccgaag gtggaaattc tgtgaataca aattcacaat tcattaagca tcagcgaact
      601 caaaacatag ataaacccca tgtatgcact gagtgtggga aggctttcct caagaagtct
      661 cgcctcatct atcatcagag agttcacact ggggagaaac ctcatggatg cagtatatgt
      721 gggaaagcct tctccagaaa gtccgggctc actgaacacc agagaaacca cacaggagag
      781 aaaccctatg aatgcactga atgtgacaaa gcattccgct ggaaatcaca gctcaatgca
      841 caccagaaaa ttcatacagg agagaagtca tatatatgca gtgattgtgg aaaaggcttc
      901 atcaagaagt ctcggctcat taatcatcag agagttcata caggagagaa accacatgga
      961 tgcagcctgt gtgggaaggc cttctccaaa aggtccaggc tcactgaaca ccagagaact
     1021 catacaggag agaaacccta tgaatgcact gaatgtgaca aagcattccg ctggaaatca
     1081 cagctcaatg cacatcagaa agctcacaca ggagagaagt catatatatg ccgtgattgt
     1141 ggaaaaggct tcattcagaa gggaaatctc attgtacatc agcgaattca tactggagaa
     1201 aaaccctata tatgcaatga atgtggaaaa ggcttcatcc aaaagggcaa cctccttatt
     1261 catcgacgta ctcacactgg agagaaaccc tatgtatgca atgaatgtgg gaaaggcttc
     1321 agccagaaga catgtttaat atcccatcag agatttcaca caggaaagac accctttgta
     1381 tgtactgagt gtggaaaatc ctgctcacac aagtcaggtc tcattaacca ccagagaatt
     1441 cacacaggag agaaacccta tacatgcagt gactgtggga aagctttcag agataaatca
     1501 tgtctcaaca gacatcggag aactcataca ggggagagac cgtatggatg ctctgattgt
     1561 gggaaagctt tctcccactt gtcatgcctt gtttatcata agggaatgct gcatgcaaga
     1621 gagaaatgtg taggttcagt caaattggaa aatccttgct cagagagtca tagcttatca
     1681 catacacgtg atctcataca ggataaagac tctgttaaca tggtgactct gcagatgcct
     1741 tctgtggcag ctcagacctc attaactaac agtgcgttcc aagcagagag caaagtagcc
     1801 attgtgagcc agcctgttgc cagaagttca gtctcagcag atagtagaat ttgcacagaa
     1861 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1921 ggaaaggacg aatggcgata tcaagtgctt aggat
//
