LOCUS       sequence-283788-        2660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0579
FEATURES             Location/Qualifiers
     source          1..2660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccact gacatttaaa gatgtcgcca tagaattctc tctggaggag
       61 tggcaatgcc tggatactgc acagcgggat ttatatagga atgtgttgtt agagaactac
      121 agaaacctgg tctttttggg tattgctgtc tctaagccat atctgatcac ctgtctggag
      181 caaaaaaaag agccctggaa tataaaaaga catgagatgg tagccaaacc cccagtaatg
      241 tcttttcatt ttgcccaaga cctttggcca gagcagaaca taaaagattc tttccagaaa
      301 gtgacactga gaagatacgg aaaatgtgaa tatgagaatt tacagttaag aaaaggctgt
      361 aaacatgtgg atgagtgtac ggggcacaaa ggaggtcata atacagttaa ccaatgtttg
      421 acagctaccc caagcaaaat attccagtgt aataaatatg tgaaagtctt tgataaattt
      481 tcaaattcaa atagatataa gagaagacat acaggaaaca aacacttcaa atgtaaagaa
      541 tgtagcaaat cattttgcgt gctttcacaa ctaactcagc atagaagaat tcatactaga
      601 gtgaattcct acaaatgtga agaatgtgga aaagccttta actggttctc aactcttact
      661 aaacataaga gaattcatac tggagaaaag ccctacaaat gtgaagaatg tggcaaagcc
      721 tttaaccagt cctcacaact tactaggcat aagataattc atactgaaga gaaacccaac
      781 aaatgtgaag aatgtggcaa ggcctttaaa caggcctcac accttactat acataaaata
      841 attcatactg gagaaaaacc ttacaaatat gaagaatgtg gcaaagtctt tagccagtcc
      901 tcacacctta ctacacaaaa gatacttcac actggagaga acctctacaa gtgtaaagaa
      961 tgtggaaaag cttttaacct attctcaaat cttactaacc ataagagaat tcatgctggg
     1021 gagaaaccct acaaatgtaa agaatgtggc agagctttta acatatcctc aaaccttaat
     1081 aaacaggaga aaattcatac tggagggaaa ctcaacaaat gtgaagaatg tgacaaagct
     1141 tttaaccgat ccttaaaact tactgcacat aagaaaattc taatggaaga gaaaccctac
     1201 aaatgtgaag aatgtggcaa agtctttaac cagttctcaa ctcttactag acataagata
     1261 attcatactg gagagaaacc ctacaaatgt aaagaatgtg gcaaagcttt taaccaatct
     1321 tcaaacctta ctgaacataa gaaaattcat actgcagaga aatcctataa atgtgaagaa
     1381 tgtggcaaag cttttaacca acactcaaac ctaattaacc ataggaaaat ttattctgga
     1441 gagaaaccat acaaatgtga agaatgtgga aaagctttta atcgatcctc aacccttact
     1501 agacataaga aaattcatac tggagagaaa ccctacaaat gtgaagaatg tgacagagct
     1561 tttagccagt cttcaaacct tactgaacat aagaaaattc atactggaga gaaaccctat
     1621 aaatgtgagg aatgtggcaa agcttttaac cgattctcaa cccttactaa acataagaga
     1681 attcatactg gagaaaaacc ctataaatgt gaagaatgtg gaaaagcctt taatcaatcc
     1741 tatcaactta ctagacataa gatagttcat actaaagaga aactcaacaa atgtgaagaa
     1801 tttggcaagg cctttaaaca gtcctcacac cgtactatac ataaaattat tcatactgga
     1861 gagaaaccct acaaatgtga agaacatggc aaagttttta accagtcctc aaaccttact
     1921 acacaaaaga taattcatac tggagagaac ctctacaaat ttgaagaaca tggaaaagct
     1981 tttaacctat tctcaaacat tactaaccat aagataattt atactggaga gaaaccccac
     2041 aaatgtgaag aatgtggaaa agcttataac cgattctcaa acctaactat acataagaga
     2101 attcatacgg gagagaaacc ttaccaatgt gcagaatgtg gcaaagcctt taactgctcc
     2161 tcaaccctta atagacataa gataattcat actggagaga aaccttacaa atgtaaagaa
     2221 tgtggcaaag cttttaacct atcctcaacc cttactgcac ataagaaaat tcatactgga
     2281 gagaaaccct ataaatgtga agaatgtggc aaagctttta accaatcctc aaaccttact
     2341 acacataaga aaattcatac ttcagagaaa ccctacaaat gtgaagaatg tggcaaatcc
     2401 tttaaccagt tctcatctct taatatacat aagataattc atactggaga gaaaccctac
     2461 aaatgtggag attatggcag agctttcaac ctatcctcaa atcttactac acataagaaa
     2521 attcatactg gagagaaacc ctacaaatgt gagtatggca aaacttagta atgaactagt
     2581 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgataag
     2641 tcggtctcca gtgtactcgt
//
