LOCUS       sequence-283791-        2219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0624
FEATURES             Location/Qualifiers
     source          1..2219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggctttat gttacctctc atcttcaggt acagcccacg cttgatggaa
       61 aaaattctcc aaatggctga aggtattgat attggggaga tgccttcata tgatctggtg
      121 ctgtccaaac cttccaaagg tcaaaaacgc cacctctcaa catgtgatgg tcaaaatcct
      181 cctaaaaagc aagccggttc caaattccat gcgagacctc gttttgagcc tgtacatttt
      241 gtagctagta gttcaaaaga tgaaagacag gaagatcctt atggccctca aacaaaagag
      301 gtaaatgaac aaacacattt tgccagcatg ccaagagaca tctaccaaga ttatactcaa
      361 gactctttca gtatacaaga tgggaattct cagtattgtg attcatcagg attcattctc
      421 acaaaagacc agcctgtaac agccaacatg tattttgaca gtgggaaccc tgccccaagc
      481 accacatcac agcaggcaaa ctctcagtca actcctgagc cttcaccatc acagacattt
      541 cccgagtctg tggtagccga gaagcagtat tttattgaaa aattaacggc gacaatctgg
      601 aagaaccttt ctaatccaga aatgacttct ggatctgata aaattaatta tacatatatg
      661 ttaactcgtt gtattcaggc gtgtaagaca aatcctgagt atatatatgc tcctttaaag
      721 gaaattcctc ctgccgacat ccccaaaaat aaaaaacttc taactgatgg ctatgcttgt
      781 gaagttagat gccaaaatat ctacttaact acaggttatg ctggcagcaa gaatgggtcc
      841 agggatcgag ctacagagct agctgtaaaa ctcttgcaga aacgtattga agttagagtt
      901 gtccggcgga aattcaagca tacatttgga gaggacctcg tggtgtgtca gattggcatg
      961 tcctcctatg aatttcctcc agctctgaag ccaccagaag acctggtggt gctgggtaaa
     1021 gatgcttccg ggcagccaat ttttaatgct tctgccaaac actggaccaa ttttgtcatt
     1081 acagaaaatg caaatgatgc aattggtatc cttaacaatt ctgcctcatt caacaagatg
     1141 tcaattgaat acaaatatga gatgatgcca aatcgcacat ggcgttgtcg agtgttttta
     1201 caagatcact gcttagctga aggttatgga accaagaaaa caagtaaaca tgcagctgcc
     1261 gacgaggctt tgaaaattct tcaaaaaaca cagcccactt atccatctgt caaaagttca
     1321 caatgccata caggctcttc acccagagga tctggaaaga agaaagatat aaaggatctt
     1381 gtagtttatg agaattcttc aaatcccgtg tgcacgctga acgacacagc tcagtttaac
     1441 cgaatgacag ttgagtatgt ctatgaaagg atgacaggcc tccgctggaa atgcaaagtg
     1501 attctagaga gtgaagtaat tgcagaagca gttggggtga agaaaactgt caaatatgaa
     1561 gctgctgggg aagctgtgaa aaccctcaaa aagacccagc caactgtcat taacaacttg
     1621 aagaaaggag ctgttgaaga tgtgatttca agaaatgaaa ttcagggccg ctcagcagag
     1681 gaggcttaca aacagcaaat caaagaagat aatattggaa atcagctgct gagaaagatg
     1741 ggttggactg gtggtggttt aggtaaatct ggtgagggca tacgggagcc tatctcagtg
     1801 aaagagcagc ataagcggga agggcttggt ctggatgtag agagggtgaa taaaattgcc
     1861 aagagagata ttgaacagat catcagaaac tacgcccgct ccgagagcca cacagatttg
     1921 actttctcta gagagctgac taatgatgaa cggaagcaaa tacatcagat tgcccagaag
     1981 tatggtctta agagtaagtc tcatggggtg ggccatgata ggtacctagt ggtaggtaga
     2041 aaaagacgga aggaagacct actagatcag ctcaaacagg aaggccaagt gggccattac
     2101 gagcttgtta tgcctcaagc aaattagtaa tgaactagtc cgtaacttga aagtatttcg
     2161 atttcttggc tttatatatc ttgtggaaag gacgagggcg taagtacaac atcgaatcg
//
