LOCUS       sequence-283794-        7115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0707
FEATURES             Location/Qualifiers
     source          1..7115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgtcaagttc aggttatcct cccaaccaag gagcattcag cacagaacaa
       61 agtcgttatc ctcctcactc tgtccagtat acatttccca acacccgcca ccagcaggag
      121 ttcgcagtcc ctgattatcg ttcctctcat cttgaagtga gtcaggcatc acagcttttg
      181 cagcaacagc agcagcaaca gcttcgaagg cgaccttcct tgctttcaga atttcaccca
      241 ggttctgaca ggcctcaaga aaggagaact agttatgaac cgtttcatcc aggcccatcc
      301 ccagtggatc atgattcact ggaatcgaag cgaccacgtc tggaacaggt ttctgattct
      361 cattttcagc gtgtcagtgc tgcggttttg cctttagtgc acccgctgcc agaagggctg
      421 agggcttctg cagatgctaa gaaggatcca gcattcggag gcaaacatga agctccatcc
      481 tctccaattt cggggcaacc atgtggagat gatcaaaatg cttcaccttc aaaactctca
      541 aaggaagagt taatacagag tatggatcgt gtagatcgag aaattgcaaa agtagaacag
      601 cagatcctta aactgaaaaa gaaacaacaa cagcttgaag aagaggcagc taaacctcct
      661 gagcctgaga agcccgtgtc ccctcctcct gtggagcaga aacaccgcag tattgtccaa
      721 attatttatg atgagaatcg gaaaaaagca gaagaagctc ataaaatttt tgaaggtctt
      781 ggcccaaaag ttgaactgcc actgtataac cagccatcag ataccaaggt gtaccatgag
      841 aacatcaaga caaaccaggt gatgaggaaa aaactcattt tattttttaa aagaagaaat
      901 catgcaagaa aacaaaggga acaaaaaatc tgccagcgtt atgatcagct catggaggca
      961 tgggagaaaa aagtggacag aatagaaaat aatcctcgga ggaaagctaa agaaagcaaa
     1021 acaagggaat actatgaaaa gcagtttcca gaaattcgaa aacaaagaga acagcaagaa
     1081 agatttcagc gagttgggca gaggggagct ggtctttcag ccaccattgc taggagtgag
     1141 catgagattt ctgaaattat tgatgggctc tctgagcagg agaataatga gaaacaaatg
     1201 cggcagctct ctgtgattcc acctatgatg tttgatgcag aacaaagacg agtcaagttc
     1261 attaacatga atgggcttat ggaggaccct atgaaagtgt ataaagatag gcagtttatg
     1321 aatgtttgga ctgaccatga aaaggagatc tttaaggaca agtttatcca gcatccaaaa
     1381 aactttggac taattgcatc atacttggag aggaagagtg ttcctgattg tgttttgtat
     1441 tactatttaa ccaagaaaaa tgagaattat aaagccctcg tcagaaggaa ttatgggaaa
     1501 cgcagaggca gaaaccagca aattgctcga ccctcgcaag aagaaaaagt agaagaaaaa
     1561 gaagaggata aagcagaaaa aacagaaaaa aaagaagaag aaaagaaaga tgaagaggaa
     1621 aaagatgaaa aagaagactc caaagaaaat accaaggaaa aggacaagat agatggtaca
     1681 gcagaagaaa ctgaggaaag agagcaagcc acaccccggg ggcgaaagac tgccaacagt
     1741 cagggccgcc gtaagggccg gatcaccagg tccatgacaa acgaagctgc agctgccagt
     1801 gctgcagccg cagcggctac tgaagagccc ccaccacctc tgccaccgcc accagaaccc
     1861 atttctacag agcctgtgga gacctctcga tggacagaag aagaaatgga agttgctaaa
     1921 aaaggtctag tagaacatgg tcgtaactgg gcagcaattg ctaaaatggt gggaacgaaa
     1981 agtgaagctc aatgtaaaaa cttctatttt aactataaaa ggcgacacaa tcttgacaac
     2041 ctcttacagc agcataaaca gaaaacttca cgaaaacctc gtgaagagcg agatgtgtct
     2101 caatgtgaaa gtgtcgcttc cactgtttct gctcaggagg atgaagatat tgaagcctcc
     2161 aatgaagaag aaaatccaga agacagcgaa ggtgcagaaa atagttctga tacagaaagt
     2221 gctccttctc cttcaccagt tgaagctgtc aagcccagcg aggacagtcc tgaaaatgct
     2281 acttctcgag gaaacacaga acctgcggtt gagcttgagc ccaccacgga aactgcaccc
     2341 agtacatctc cctccttagc agttccaagt acaaaaccag ctgaagatga aagtgtggag
     2401 acccaggtga atgacagcat cagtgctgag acagcagagc agatggatgt agatcagcag
     2461 gagcacagtg ctgaagaggg ttctgtttgt gatcccccac ccgctaccaa agctgactct
     2521 gtggacgttg aagtgagggt gccagaaaac catgcatcta aagttgaagg tgataatacc
     2581 aaagaaagag acttggatag agccagtgag aaggtggaac ctagagatga agatttggtg
     2641 gtagctcagc aaataaatgc ccaaaggccc gagccccagt cagacaatga ttccagtgcc
     2701 acgtgcagcg ctgatgagga tgtggatgga gagccagaga ggcagagaat gtttcctatg
     2761 gactcaaagc cttcactgtt aaaccccact ggatctatac tcgtctcatc tccgttaaaa
     2821 ccaaatccac tggatctgcc acagcttcag catcgagctg ctgttatccc accaatggta
     2881 tcctgcaccc catgtaacat accaattgga accccagtga gcggctatgc tctctaccag
     2941 cgacacatta aagcaatgca tgagtcagca ctcctggagg agcagcggca gagacaagaa
     3001 cagatagatt tggaatgtag aagttctaca agtccatgtg gcacatccaa gagtccaaac
     3061 agagagtggg aagtccttca gcctgctcca catcaagtga taactaatct ccctgaaggc
     3121 gttcggcttc cgacaactcg accaaccagg ccaccgcccc ctctcatccc gtcatccaaa
     3181 accacagtgg cttcagaaaa accatctttt ataatgggag gctccatctc acagggaaca
     3241 ccaggcactt atttgacttc tcataatcag gcttcctaca ctcaagaaac acccaagccg
     3301 tcagtgggat ctatctctct tggactgcca cggcaacagg aatctgccaa atcagctact
     3361 ttgccctaca tcaagcagga agaattttct ccccgaagcc aaaactcaca acctgagggt
     3421 ctgttggtca gggcccaaca tgaaggtgta gtcagaggta ccgcaggagc catacaagaa
     3481 ggaagtataa ctcggggaac tccaaccagc aaaatttcag tggagagcat tccatcccta
     3541 cggggctcta tcactcaggg caccccggct ctgccccaga ctggcatacc aacagaggct
     3601 ttggtgaagg ggtccatttc gagaatgccc attgaagaca gcagtcctga gaaaggcaga
     3661 gaggaagctg catccaaagg ccatgttatt tatgaaggca aaagtggaca tatcttgtca
     3721 tatgataata ttaagaatgc ccgagaaggg actaggagtc caagaacagc tcatgaaatc
     3781 agtttaaaga gaagctatga atcagtggaa ggaaatataa agcaagggat gtcaatgagg
     3841 gagtctcctg tatcagcacc gttagagggg ctgatatgcc gagcattacc cagggggagt
     3901 cctcattctg acctcaaaga aaggactgta ttgtctggct ccataatgca ggggacacca
     3961 agagcaacaa ctgaaagctt tgaagatggc cttaaatatc ccaaacaaat taaaagggaa
     4021 agtcctccca tacgagcatt tgaaggtgcc attaccaaag gaaaaccata tgatggcatc
     4081 accaccatca aagaaatggg gcgttccatt catgagattc caaggcaaga tattttaact
     4141 caggaaagtc ggaaaactcc agaagtggtc cagagcacac ggccgataat tgagggttcc
     4201 atttcccagg gcacaccaat aaagtttgac aacaactcag gtcaatctgc catcaaacac
     4261 aatgtcaaat ccttaatcac ggggcctagc aaactatccc gtggaatgcc tccgctggaa
     4321 attgtgccag agaacataaa agtggtagaa cggggaaaat atgaggatgt gaaagcaggc
     4381 gagaccgtgc gttcccggca cacgtcagtg gtaagctctg gcccctccgt tcttaggtcc
     4441 acactgcatg aagctcccaa agcacaactg agccctggga tttatgatga caccagtgca
     4501 cggaggaccc ctgtgagtta tcaaaacacc atgtccagag gctcacccat gatgaacaga
     4561 acttctgatg ttacaatttc ttctaacaag tctaccaatc atgaaaggaa atcgacactg
     4621 acccctaccc agagggaaag tatcccagcg aagtctccag tgcctggggt ggaccctgtc
     4681 gtgagccaca gtccgtttga tccccatcac agaggcagca ctgcaggcga ggtttatcgg
     4741 agccacctgc ccacgcactt ggatccagcc atgccttttc acagggcttt ggatcctgca
     4801 gcggctgctt acctgtttca gagacagctt tcaccaactc caggttaccc aagtcagtat
     4861 cagctttacg caatggagaa cacaagacag acaatcttaa atgattacat tacctcacaa
     4921 cagatgcaag tgaacttgcg tccagatgtg gccagaggac tctccccaag agagcagcca
     4981 ctgggtctcc catacccagc aacgagagga atcattgacc tgaccaatat gcctccaaca
     5041 attttagtgc ctcatccagg gggaacaagc actcctccca tggacagaat cacttatatt
     5101 cctggtacac agattacttt ccctcccagg ccgtacaact ctgcttccat gtctccagga
     5161 cacccaacac accttgcagc tgctgcaagt gctgagaggg aacgggaacg ggagcgggag
     5221 aaggagcggg agcgggaacg gattgctgca gcttcctccg acctctacct gcggccaggc
     5281 tcagaacagc ctggccgacc tggcagtcat ggatatgttc gctccccttc cccttcagta
     5341 agaactcagg agaccatgtt gcaacagaga cccagtgttt tccaaggaac caatggaacc
     5401 agtgtaatca cacctttgga tccaactgct cagctacgaa tcatgccact gcctgctggg
     5461 ggcccttcaa taagccaagg cctgccagcc tcccgttaca acactgctgc ggatgccctg
     5521 gctgctcttg tggatgctgc agcttctgca ccccagatgg atgtgtccaa aacaaaagag
     5581 atatcttctc acaggtatga aacacctagc gatgctattg aggtgataag tcctgccagc
     5641 tcacctgcgc caccccagga gaaactgcag acctatcagc cagaggttgt taaggcaaat
     5701 caagcggaaa atgatcctac cagacaatat gaaggaccat tacatcacta tcgaccacag
     5761 caggaatcac catctcccca acaacagctg cccccttctt cacaggcaga gggaatgggg
     5821 caagtgccca ggacccatcg gctgatcaca cttgctgatc acatctgtca aattatcaca
     5881 caagattttg ctagaaatca agtttcctcg cagactcccc agcagcctcc tacttctaca
     5941 ttccagaact caccttctgc tttggtatct acacctgtga ggactaaaac atcaaaccgt
     6001 tacagcccag aatcccaggc tcagtctgtc catcatcaaa gaccaggttc aagggtctct
     6061 ccagaaaatc ttgtggacaa atccagggga agtaggcctg gaaaatcccc agagaggagt
     6121 cacgtctctt cggagcccta cgagcccatc tccccacccc aggttccggt tgtgcatgag
     6181 aaacaggaca gcttgctgct cttgtctcag aggggcgcag agcctgcaga gcagaggaat
     6241 gatgcccgct caccagggag tataagctac ttgccttcat tcttcaccaa gcttgaaaat
     6301 acatcaccca tggttaaatc aaagaagcag gagatttttc gtaagttgaa ctcctctggt
     6361 ggaggtgact ctgatatggc agctgctcag ccaggaactg agatctttaa tctgccagca
     6421 gttactacgt caggctcagt tagctctaga ggccattctt ttgctgatcc tgccagtaat
     6481 cttgggctgg aagacattat caggaaggct ctcatgggaa gctttgatga caaagttgag
     6541 gatcatggag ttgtcatgtc ccagcctatg ggagtagtgc ctggtactgc caacacctca
     6601 gttgtgacca gtggtgagac acgaagagag gaaggggacc catcacctca ttcaggagga
     6661 gtttgcaaac caaagctgat cagcaagtca aacagcagga aatctaagtc tcctatacct
     6721 gggcaaggct acttaggaac ggaacggccc tcttcagtct cctctgtaca ttcagaaggg
     6781 gattaccata ggcagacgcc agggtgggcc tgggaagaca ggccctcttc aacaggctca
     6841 actcagtttc cttataaccc tctgactatg cggatgctca gcagtactcc accaacaccg
     6901 attgcatgtg ctccctctgc ggtgaaccaa gcagctcctc accaacagaa caggatctgg
     6961 gagcgagagc ctgccccact gctctcagca cagtacgaga ccctgtcgga tagtgatgac
     7021 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     7081 ggaaaggacg actggtaatc gcgcataacc aaaca
//
