LOCUS       sequence-28380-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP30
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatgaccg cggccgacag
      121 ggccatccag cgcttcctgc ggaccggggc ggccgtcaga tataaagtca tgaagaactg
      181 gggagttata ggtggaattg ctgctgctct tgcagcagga atatatgtta tttggggtcc
      241 cattacagaa agaaagaagc gtagaaaagg gcttgtgcct ggccttgtta atttagggaa
      301 cacctgcttc atgaactccc tgctacaagg cctgtctgcc tgtcctgctt tcatcaggtg
      361 gctggaagag ttcacctccc agtactccag ggatcagaag gagcccccct cacaccagta
      421 tttatcctta acactcttgc accttctgaa agccttgtcc tgccaagaag ttactgatga
      481 tgaggtctta gatgcaagct gcttgttgga tgtcttaaga atgtacagat ggcagatctc
      541 atcatttgaa gaacaggatg ctcacgaatt attccatgtc attacctcgt cattggaaga
      601 tgagcgagac cgccagcctc gggtcacaca tttgtttgat gtgcattccc tggagcagca
      661 gtcagaaata actcccaaac aaattacctg ccgcacaaga gggtcacctc accccacatc
      721 caatcactgg aagtctcaac atccttttca tggaagactc actagtaata tggtctgcna
      781 acactgtgaa caccagantc ct
//
