LOCUS       sequence-283800-        3035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0864
FEATURES             Location/Qualifiers
     source          1..3035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccaagt tgcaatgtcc accctccccg ttgaagatga ggagtcctcg
       61 gagagcagga tggtggtgac attcctcatg tcagctctcg agtccatgtg taaagaactg
      121 gccaagtcca aagccgaagt ggcctgcatt gcagtgtatg aaacagacgt gtttgtcgtc
      181 ggaactgaaa gaggacgtgc ttttgtcaat accagaaagg attttcaaaa agattttgta
      241 aaatattgtg ttgaagaaga agaaaaagct gcagagatgc ataaaatgaa atctacaacc
      301 caggcaaatc ggatgagtgt agatgctgta gaaattgaaa cactcagaaa aacagttgag
      361 gactatttct gcttttgcta tgggaaagct ttaggcaaat ccacagtggt acctgtacca
      421 tatgagaaga tgctgcgaga ccagtcggct gtggtagtgc aggggcttcc ggaaggtgtt
      481 gcctttaaac accccgagaa ctatgatctt gcaaccctga aatggatttt ggagaacaaa
      541 gcagggattt cattcatcat taagagacct tttttagagc caaagaagca tgtaggtggt
      601 cgtgtgatgg taacagatgc tgacaggtca atactatctc caggtggaag ttgtggcccc
      661 atcaaagtga aaactgaacc cacagaagat tctggcattt ccctggaaat ggcagctgtg
      721 acagtaaagg aagaatcaga agatcctgat tattatcaat ataacattca agcaggccct
      781 tctgaaactg atgatgttga tgaaaaacag cccctatcga agcctttgca aggaagccac
      841 cattcttcag agggcaatga aggcacagaa atggaagtac cagcagaaga tgatgattat
      901 tctccaccgt ctaagagacc aaaggccaat gagctaccgc agccaccagt cccggaaccc
      961 gccaatgctg ggaagcggaa agtgagggag ttcaacttcg agaaatggaa tgctcgcatc
     1021 actgatctac gtaaacaagt tgaagaattg tttgaaagga aatatgctca agccataaaa
     1081 gccaaaggtc cggtgacgat cccgtaccct cttttccagt ctcatgttga agatctttat
     1141 gtagaaggac ttcctgaagg aattcctttt agaaggccat ctacttacgg aattcctcgc
     1201 ctggagagga tattacttgc aaaggaaagg attcgttttg tgattaagaa acatgagctt
     1261 ctgaattcaa cacgtgaaga tttacagctt gataagccag cttcaggagt aaaggaagaa
     1321 tggtatgcca gaatcactaa attaagaaag atggtggatc agcttttctg caaaaaattt
     1381 gcggaagcct tggggagcac tgaagccaag gctgtaccgt accaaaaatt tgaggcacac
     1441 ccgaatgatc tgtacgtgga aggactgcca gaaaacattc ctttccgaag tccctcatgg
     1501 tatggaatcc caaggctgga aaaaatcatt caagtgggca atcgaattaa atttgttatt
     1561 aaaagaccag aacttctgac tcacagtacc actgaagtta ctcagccaag aacgaataca
     1621 ccagtcaaag aagattggaa tgtcagaatt accaagctac ggaagcaagt ggaagagatt
     1681 tttaatttga aatttgctca agctcttgga ctcaccgagg cagtaaaagt accatatcct
     1741 gtgtttgaat caaacccgga gttcttgtat gtggaaggct tgccagaggg gattcccttc
     1801 cgaagcccta cctggtttgg aattccacga cttgaaagga tcgtccgcgg gagtaataaa
     1861 atcaagttcg ttgttaaaaa acctgaacta gttatttcct acttgcctcc tgggatggct
     1921 agtaaaataa acactaaagc tttgcagtcc cccaaaagac cacgaagtcc tgggagtaat
     1981 tcaaaggttc ctgaaattga ggtcaccgtg gaaggcccta ataacaacaa tcctcaaacc
     2041 tcagctgttc gaaccccgac ccagactaac ggttctaacg ttcccttcaa gccacgaggg
     2101 agagagtttt cctttgaggc ctggaatgcc aaaatcacgg acctaaaaca gaaagttgaa
     2161 aatctcttca atgagaaatg tggggaagct cttggcctta aacaagctgt gaaggtgccg
     2221 ttcgcgttat ttgagtcttt cccggaagac ttttatgtgg aaggcttacc tgagggtgtg
     2281 ccattccgaa gaccatcgac ttttggcatt ccgaggctgg agaagatact cagaaacaaa
     2341 gccaaaatta agttcatcat taaaaagccc gaaatgtttg agacggcgat taaggagagc
     2401 acctcctcta agagccctcc cagaaaaata aattcatcac ccaatgttaa tactactgca
     2461 tcaggtgttg aagaccttaa catcattcag gtgacaattc cagatgatga taatgaaaga
     2521 ctctcgaaag ttgaaaaagc tagacagcta agagaacaag tgaatgacct ctttagtcgg
     2581 aaatttggtg aagctattgg tatgggtttt cctgtgaaag ttccctacag gaaaatcaca
     2641 attaaccctg gctgtgtggt agttgatggc atgcccccgg gggtgtcctt caaagccccc
     2701 agctacctgg aaatcagctc catgagaagg atcttagact ctgccgagtt tatcaaattc
     2761 acggtcatta gaccatttcc aggacttgtg attaataacc agctggttga tcagagtgag
     2821 tcagaaggcc ccgtgataca agaatcagct gaaccaagcc agttggaagt tccagccaca
     2881 gaagaaataa aagagactga tggaagctct cagatcaagc aagaaccaga ccccacgtgg
     2941 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3001 ggaaaggacg aggcagcagg agcttgcgcg cttgt
//
