LOCUS       sequence-283806-        2858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0909
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtcgctc caattctaga tcacattctt caaggtcaaa gtctagatca
       61 cagtctagtt ctcgatcaag atcaagatct cattctagaa agaagcgata caggtctcgt
      121 tccagaacat attcaaggtc tcgtagtaga gatcgtatgt attctagaga ttatcgtcgc
      181 gattacagaa ataatagagg aatgagacga ccttatgggt acagaggaag gggtagaggg
      241 tattatcaag gaggaggagg tagatatcat cgaggtggtt atagacctgt ctggaataga
      301 aggcactcta ggagtcctag acgaggtcgt tcacgttcca ggagtccaaa aagaagatcc
      361 gtttcttctc aaagatccag aagcagatct cgccggtcat atagatcttc taggtctcca
      421 agatcatcct cttctcgttc ttcatcccca tatagcaaat ctcctgtttc taaaagacga
      481 gggtctcagg aaaaacaaac caaaaaagct gaaggggaac cccaagaaga gagtccgttg
      541 aaaagtaaat cacaggagga accgaaagat acatttgaac atgacccatc tgagtctatc
      601 gatgaattta ataagtcatc agccacatcc ggtgatattt ggcctggcct ttcagcttat
      661 gataatagtc ctagatcacc ccatagtcct tcacctattg ctacaccacc tagtcagagt
      721 tcatcttgct ctgatgctcc catgctcagt acagttcact ctgcaaaaaa tactccttct
      781 cagcattcac attccattca gcatagtcct gaaaggtctg ggtctggttc tgttggaaat
      841 ggatctagtc gatacagtcc ttctcagaat agtccaattc atcacatccc ttcacgaaga
      901 agtcctgcaa agacaatcgc accacagaat gctccaagag atgagtctag gggccgttcc
      961 tcgttttatc ctgatggtgg agatcaggaa actgcaaaga ctgggaagtt cttaaaaagg
     1021 ttcacagatg aagagtctag agtattcctg cttgataggg gtaataccag ggataaagag
     1081 gcttcaaaag agaaaggatc agagaaaggg agggcagagg gagaatggga agatcaggaa
     1141 gctctagatt acttcagtga taaagagtct ggaaaacaaa agtttaatga ttcagaaggg
     1201 gatgacacag aggagacaga ggattataga cagttcagga agtcagtcct cgcagatcag
     1261 ggtaaaagtt ttgctactgc atctcaccgg aatactgagg aggaaggact caagtacaag
     1321 tccaaagttt cactgaaagg caatagagaa agtgatggat ttagagaaga aaaaaattat
     1381 aaacttaaag agactggata tgtagtggaa aggcctagca ctacaaaaga taagcacaaa
     1441 gaagaagaca aaaattctga aagaataaca gtaaagaaag aaactcagtc acctgagcag
     1501 gtaaagtctg aaaagctcaa agacctcttt gattacagtc cccctctaca caagaatctg
     1561 gatgcacgag aaaagtctac cttcagagag gaaagcccac ttaggatcaa aatgatagcg
     1621 agtgattctc accgtcctga agtcaaactc aaaatggcac ctgttcctct tgatgattct
     1681 aacagacctg cttccttgac taaagacagg ctgcttgcta gtacacttgt ccattctgtc
     1741 aagaaggagc aagaattccg atccatcttt gaccacatta agttgccaca ggccagcaaa
     1801 agcacttcag agtcatttat tcaacacatt gtgtccttgg ttcatcatgt taaagagcaa
     1861 tacttcaagt cagctgcaat gaccctaaac gagcggttca cttcgtatca gaaagccact
     1921 gaagaacata gtactcggca aaagagccct gaaatacaca ggagaattga catctcacca
     1981 agtaccctga ggaagcatac ccgtttagca ggggaagaga gagtttttaa agaagaaaat
     2041 caaaagggag ataaaaaatt aaggtgtgac tctgctgacc ttcggcatga cattgatcgc
     2101 cgtagaaaag aaagaagtaa agaacgggga gattccaagg gctccaggga atccagtgga
     2161 tcaagaaagc aggaaaaaac tccaaaagat tacaaggaat acaaatctta caaagatgac
     2221 agtaaacata aaagagagca agatcattct cgatcttcat cctcttcagc atcaccttct
     2281 tctcccagtt ctcgagaaga aaaggagagt aagaaggaaa gagaagaaga atttaaaact
     2341 caccatgaaa tgaaagaata ctcaggcttt gcaggagtta gccgaccacg aggaaccttt
     2401 tttcgaatta gaggcagagg aagagccaga ggagtttttg ctgggacaaa tactggtcca
     2461 aacaactcaa atactacttt tcaaaagaga ccgaaggaag aggaatggga tccagaatat
     2521 accccaaaga gcaagaagta cttcttgcat gacgacagag atgatggtgt ggattattgg
     2581 gccaaaagag gaagaggtcg tggtactttt caacgtggca gagggcgctt taacttcaaa
     2641 aaatcaggta gcagtcctaa atggactcat gacaaatacc aaggggatgg gattgttgaa
     2701 gatgaagaag agaccatgga aaataatgaa gaaaagaagg acagacgcaa ggaagaaaag
     2761 gaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2821 tgtggaaagg acgatttacc gacggatgat tgaagtta
//
