LOCUS       sequence-283809-        4850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0952
FEATURES             Location/Qualifiers
     source          1..4850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggttccaa gagaagaaga gctacctccc cttccagcag tgtcagcggg
       61 gactttgatg atgggcacca ttctgtgtca acaccaggcc caagcaggaa aaggaggaga
      121 ctttccaatc ttccaactgt agatcctatt gccgtgtgcc atgaactcta taataccatc
      181 cgagactata aggatgaaca gggcagactt ctctgtgagc tcttcattag ggcaccaaag
      241 cgaagaaatc aaccagacta ttatgaagtg gtttctcagc ccattgactt gatgaaaatc
      301 caacagaaac taaaaatgga agagtatgat gatgttaatt tgctgactgc tgacttccag
      361 cttcttttta acaatgcaaa gtcctattat aagccagatt ctcctgaata taaagccgct
      421 tgcaaactct gggatttgta ccttcgaaca agaaatgagt ttgttcagaa aggagaagca
      481 gatgacgaag atgatgatga agatgggcaa gacaatcagg gcacagtgac tgaaggatct
      541 tctccagctt acttgaagga gatcctggag cagcttcttg aagccatagt tgtagctaca
      601 aatccatcag gacgtctcat tagcgaactt tttcagaaac tgccttctaa agtgcaatat
      661 ccagattatt atgcaataat taaggagcct atagatctca agaccattgc ccagaggata
      721 cagaatggaa gctacaaaag tattcatgca atggccaaag atatagatct cctcgcaaaa
      781 aatgccaaaa cttataatga gcctggctct caagtattca aggatgcaaa ttcaattaaa
      841 aaaatatttt atatgaaaaa ggctgaaatt gaacatcatg aaatggctaa gtcaagtctt
      901 cgaatgagga ctccatccaa cttggctgca gccagactga caggtccttc acacagtaaa
      961 ggcagccttg gtgaagagag aaatcccact agcaagtatt accgtaataa aagagcagta
     1021 caaggaggtc gtttatcagc aattacaatg gcacttcaat atggctcaga aagtgaagaa
     1081 gatgctgctt tagctgctgc acgctatgaa gagggagagt cagaagcaga aagcatcact
     1141 tcctttatgg atgtttcaaa tcctttttat cagctttatg acacagttag gagttgtcgg
     1201 aataaccaag ggcagctaat agctgaacct ttttaccatt tgccttcaaa gaaaaaatac
     1261 cctgattatt accagcaaat taaaatgccc atatcactac aacagatccg aacaaaactg
     1321 aagaatcaag aatatgaaac tttagatcat ttggagtgtg atctgaattt aatgtttgaa
     1381 aatgccaaac gctataatgt gcccaattca gccatctaca agcgagttct aaaattgcag
     1441 caagttatgc aggcaaagaa gaaagagctt gccaggagag acgatatcga ggacggagac
     1501 agcatgatct cttcagccac ctctgatact ggtagtgcca aaagaaaaag taaaaagaac
     1561 ataagaaagc agcgaatgaa aatcttattc aatgttgttc ttgaagctcg agagccaggt
     1621 tcaggcagaa gactttgtga cctatttatg gttaaaccat ccaaaaagga ctatcctgat
     1681 tattataaaa tcatcttgga gccaatggac ttgaaaataa ttgagcataa catccgcaat
     1741 gacaaatatg ctggtgaaga gggaatgata gaagacatga agctgatgtt ccggaatgcc
     1801 aggcactata atgaggaggg ctcccaggtt tataatgatg cacatatcct ggagaagtta
     1861 ctcaaggaga aaaggaaaga gctgggccca ctgcctgatg atgatgacat ggcttctccc
     1921 aaactcaagc tgagtaggaa gagtggcatt tctcctaaaa aatcaaaata catgactcca
     1981 atgcagcaga aactaaatga ggtctatgaa gctgtaaaga actatactga taagaggggt
     2041 cgccgcctca gtgccatatt tctgaggctt ccctctagat ctgagttgcc tgactactat
     2101 ctgactatta aaaagcccat ggacatggaa aaaattcgaa gtcacatgat ggccaacaag
     2161 taccaagata ttgactctat ggttgaggac tttgtcatga tgtttaataa tgcctgtaca
     2221 tacaatgagc cggagtcttt gatctacaaa gatgctcttg ttctacacaa agtcctgctt
     2281 gaaacacgca gagacctgga gggagatgag gactctcatg tcccaaatgt gactttgctg
     2341 attcaagagc ttatccacaa tctttttgtg tcagtcatga gtcatcagga tgatgaggga
     2401 agatgctaca gcgattcttt agcagaaatt cctgctgtgg atcccaactt tcctaacaaa
     2461 ccacccctta catttgacat aattaggaag aatgttgaaa ataatcgcta ccgtcggctt
     2521 gatttatttc aagagcatat gtttgaagta ttggaacgag caagaaggat gaatcggaca
     2581 gattcagaaa tatatgaaga tgcagtagaa cttcagcagt tttttattaa aattcgtgat
     2641 gaactctgca aaaatggaga gattcttctt tcaccggcac tcagctatac cacaaaacat
     2701 ttgcataatg atgtggagaa agagagaaag gaaaaattgc caaaagaaat agaggaagat
     2761 aaactaaaac gagaagaaga aaaaagagaa gctgaaaaga gtgaagattc ctctggtgct
     2821 gcaggcctct caggcttaca tcgcacatac agccaggact gtagctttaa aaacagcatg
     2881 taccatgttg gagattacgt ctatgtggaa cctgcagagg ccaacctaca accacatatc
     2941 gtctgtattg aaagactgtg ggaggattca gctgaaaaag aagtttttaa gagtgactat
     3001 tacaacaaag ttccagttag taaaattcta ggcaagtgtg tggtcatgtt tgtcaaggaa
     3061 tactttaagt tatgcccaga aaacttccga gatgaggatg tttttgtctg tgaatcacgg
     3121 tattctgcca aaaccaaatc ttttaagaaa attaaactgt ggaccatgcc catcagctca
     3181 gtcaggtttg tccctcggga tgtgcctctg cctgtggttc gcgtggcctc tgtatttgca
     3241 aatgcagata aaggtgatga tgagaagaat acagacaact cagaggacag tcgagctgaa
     3301 gacaatttta acttggaaaa ggaaaaagaa gatgtccctg tggaaatgtc caatggtgaa
     3361 ccaggttgcc actactttga gcagctccat tacaatgaca tgtggctgaa ggttggcgac
     3421 tgtgtcttca tcaagtccca tggcctggtg cgtcctcgtg tgggcagaat tgaaaaagta
     3481 tgggttcgag atggagctgc atatttttat ggccccatct tcattcaccc agaagaaaca
     3541 gagcatgagc ccacaaaaat gttctacaaa aaagaagtat ttctgagtaa tctggaagaa
     3601 acctgcccca tgacatgtat tctcggaaag tgtgctgtgt tgtcattcaa ggacttcctc
     3661 tcctgcaggc caactgaaat accagaaaat gacattctgc tttgtgagag ccgctacaat
     3721 gagagcgaca agcagatgaa gaaattcaaa ggattgaaga ggttttcact ctctgctaaa
     3781 gtggtagatg atgaaattta ctacttcaga aaaccaattg ttcctcagaa ggagccatca
     3841 cctttgctgg aaaagaagat ccagttgcta gaagctaaat ttgccgagtt agaaggtgga
     3901 gatgatgata ttgaagagat gggagaagaa gatagtgagt ctaccccaaa gtctgccaaa
     3961 ggcagtgcaa agaaggaagg ctccaaacgg aaaatcaaca tgagtggcta catcctgttc
     4021 agcagtgaga tgagggctgt gattaaggcc caacacccag actactcttt cggggagctc
     4081 agccgcctgg tggggacaga atggagaaat cttgagacag ccaagaaagc agaatatgaa
     4141 ggcatgatgg gtggctatcc gccaggcctt ccacctttgc agggcccagt tgatggcctt
     4201 gttagcatgg gcagcatgca gccacttcac cctggggggc ctccacccca ccatcttccg
     4261 ccaggtgtgc ctggcctccc gggcatccca ccaccgggtg tgatgaacca aggagtggcc
     4321 cctatggtag ggactccagc accaggtgga agtccatatg gacaacaggt gggagttttg
     4381 gggcctccag ggcagcaggc accacctcca tatcccggcc cacatccagc tggaccccct
     4441 gtcatacagc agccaacaac acccatgttt gtagctcccc caccaaagac ccagcggctt
     4501 cttcactcag aggcctacct gaaatacatt gaaggactca gtgcggagtc caacagcatt
     4561 agcaagtggg atcagacact ggcagctcga agacgcgacg tccatttgtc gaaagaacag
     4621 gagagccgcc taccctctca ctggctgaaa agcaaagggg cccacaccac catggcagat
     4681 gccctctggc gccttcgaga tttgatgctc cgggacaccc tcaacattcg ccaagcatac
     4741 aacctagaaa atgtttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     4801 ctttatatat cttgtggaaa ggacgaagac tcttctcagc gaaaccacct
//
