LOCUS       sequence-28381-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP36
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcgcgccca ccatgccaat
      121 agtggataag ttgaaggagg ccctgaaacc cggccgcaag gactcggctg atgatggaga
      181 actggggaag cttcttgcct cctctgccaa gaaggtcctt ttacagaaaa tcgagttcga
      241 gccagccagc aagagcttct cctaccagct ggaggcctta aagagcaaat atgtgttgct
      301 caaccccaaa acagagggag ctagtcgcca caagagtgga gatgacccac cggccgggag
      361 acagggcagt gagcacacgt atgagagctg tggtgacgga gtcccagccc cgcagaaagt
      421 gcttttcccc acggagcgac tgtctctgag gtgggagcgg gtcttccgcg tgggcgcagg
      481 actccacaac cttggcaaca cctgctttct caatgccacc atccagtgct tgacctacac
      541 accacctcta gccaactacc tgctctccaa ggagcatgct cgcagctgcc accagggaag
      601 cttctgcatg ctgtgtgtca tgcagaacca cattgtccag gccttcgcca acagcggcaa
      661 cgccatcaag cccgtctcct tcatccgaga cctgaaaaag atcgcccgac acttccgctt
      721 tgggaaccag gaggacgcgc atgag
//
